The occurrence and transmission characteristics of airborne H9N2 avian influenza virus

被引：26

作者：

Yao, Meiling ^{[1
]}

Zhang, Xingxiao ^{[1
]}

Gao, Jing ^{[2
]}

Chai, Tongjie ^{[1
]}

Miao, Zengmin ^{[1
]}

Ma, Weiming ^{[1
]}

Qin, Mei ^{[1
]}

Li, Qinglei ^{[1
]}

Li, Xiaoxia ^{[1
]}

Liu, Jingbo ^{[1
]}

Zhang, Hongshuang ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Anim Sci & Vet Med, Res Ctr Anim Dis Control Engn, Tai An, Shandong, Peoples R China

[2] Cent Hosp Taian City, Tai An, Shandong, Peoples R China

来源：

BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT | 2011年 / 124卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

transmission; H9N2 subtype avian influenza virus; aerosol; infection; positive-negative pressure isolator; A VIRUSES; CHICKENS; EPIDEMIOLOGY; ASSAY; TIME;

D O I：

10.2376/0005-9366-124-136

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

To better understand the transmission route of H9N2 avian influenza virus (AIV), two duplicate trials were conducted to observe the process of aerosol infection and direct contact in specific pathogen free chickens. Fifteen chickens (G1) were inoculated with H9N2 AIV and housed together with another 15 chickens (G2) in the same positive-negative-pressure isolator (A). Fifteen chickens (G3) were bred in another isolator (B) which was connected with A so that air could flow unidi-rectionally from A to B. Air, oropharyngeal and cloacal swabs, and blood samples were collected for the detection of aerosolized virus, virus shedding, and seroconversion. AIV aerosols were initially detected at day 2-3 post inoculation (dpi), reaching peak concentrations at 7 dpi. Virus shedding was detected in all chickens of G2, but only in a part in G3 (Ti: 87%,T2: 80%). Antibodies were initially detected at 4-5 dpi, peaking at 14-21 dpi. The results showed that H9N2 AIV could be transmitted by both aerosol exposure and direct contact.

引用

页码：136 / 141

页数：6

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