Reverse transcription-polymerase chain reaction (RT-PCR) in flow-through micro-reactors:: Thermal and fluidic concepts

被引：23

作者：

Felbel, J. ^{[1
]}

Reichert, A. ^{[1
]}

Kielpinski, A. ^{[1
]}

Urban, M. ^{[1
]}

Henkel, T. ^{[1
]}

Haefner, N. ^{[2
]}

Duerst, M. ^{[2
]}

Weber, J. ^{[3
]}

机构：

[1] Inst Phys High Technol eV Jena, D-07745 Jena, Germany

[2] Univ Jena, Clin Obstet & Gynaecol, D-07740 Jena, Germany

[3] Analyt Jena AG, D-07745 Jena, Germany

来源：

CHEMICAL ENGINEERING JOURNAL | 2008年 / 135卷 / S298-S302期

关键词：

flow-through PCR; miniaturized PCR; PCR-chip; lab-on-a-chip;

D O I：

10.1016/j.cej.2007.07.019

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This paper presents a novel flow-through reverse transcription-polymerase chain reaction (RT-PCR) micro-reactor with optimized thermal and fluidic characteristics. We have designed a micro-reactor comprising a heating plate for different temperature zones and a fluidic chip with serpentine micro-channels. One feature of this chip system is the generation of a segmented flow for high-throughput analysis of PCR samples. We show the first successful reactions on the chip with different flow regimes and variations of PCR methods from a standard PCR to one-step RT-PCR reactions. The aim of the experiments was the detection of the HPV 16 DNA genome and of viral oncogene transcripts, respectively. Both markers are of importance in medical diagnostics. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：S298 / S302

页数：5

共 13 条

[1] Miniaturised nucleic acid analysis [J].