Information on the selenium status of several body compartments of rats from the selenium concentrations in blood fractions, hair and nails

The suitability of the selenium concentrations in blood plasma, red blood cells, hair and nails to serve as indicators for the selenium status in the main body pools and, for the chemical forms of the element ingested was investigated in an animal model. Selenium-deficient male rats, fed a methionine-supplemented low selenium diet, were replenished over a period of 3 months: by repealed oral administration of Se-75-labeled sodium selenite or L-selenamethionine in amounts equivalent to the intake from diets with selenium concentrations of 0.3 mg/kg and 2 mg/kg. As the tracer was found to reflect the selenium distribution in the main compartments, quantitative selenium analysis in whole body, liver, skeletal muscle, heart and the monitor materials in the four groups was carried out by measuring the Se-75 activity. The blood selenium level was the most suitable parameter for assessing the selenium status in the liver while the nail selenium concentration was most suited for whole body, skeletal muscle and heart. The differences in the amount and chemical form of dietary selenium were best reflected by the ratios of the selenium levels in hair and plasma or in nails and plasma. The results suggest that with adequate or high selenium intake it might be possible to obtain information on the selenium status of the main body compartments by evaluating the selenium concentrations in severeral appropriate monitor materials.