The reaction of triplet 2-methyl-1,4-naphthoquinone (menadione) with DNA and polynucleotides

被引：32

作者：

Melvin, T ^{[1
]}

Bothe, E ^{[1
]}

SchulteFrohlinde, D ^{[1
]}

机构：

[1] MAX PLANCK INST STRAHLENCHEM,D-4330 MULHEIM,GERMANY

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1996年 / 64卷 / 05期

关键词：

D O I：

10.1111/j.1751-1097.1996.tb01833.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The photoreaction of 2-methyl-1,4-naphthoquinone (MQ, menadione) with DNA and polynucleotides in argon-saturated aqueous solution (pH 7) was studied. Results from laser flash photolysis experiments indicate that triplet quinone reacts with DNA and polyA but not detectably with polyU by one-electron oxidation of the bases of the nucleic acid with formation of the radical anion of the quinone. Irradiation of argon-saturated solutions containing MQ and DNA or polynucleotides (polyU, polyA, polyG or polyC) with 334 nm light leads to an increase in molecular weight for single-stranded DNA, polyA and to a much less extent for polyU. This finding indicates crosslink formation with quantum yields in the range of 10(-5)-10(-3).

引用

页码：769 / 776

页数：8

共 49 条

[1] RADICAL OXIDATION MECHANISMS IN CELLULAR RADIOSENSITIZATION - ELECTRON-TRANSFER IN PULSE RADIOLYSIS OF AQUEOUS NUCLEOTIDE SOLUTIONS [J].