Hematopoietic capability of CD34+ cord blood cells:: A comparison with CD34+ adult bone marrow cells

被引:34
作者
Ueda, T
Yoshida, M
Yoshino, H
Kobayashi, K
Kawahata, M
Ebihara, Y
Ito, M
Asano, S
Nakahata, T
Tsuji, K
机构
[1] Univ Tokyo, Inst Med Sci, Dept Pediat Hematol Oncol, Tokyo, Japan
[2] Univ Tokyo, Inst Med Sci, Dept Hematol Oncol, Tokyo, Japan
[3] Nippon Med Sch, Dept Pediat, Tokyo 113, Japan
[4] Hokkaido Univ, Sch Med, Dept Pediat, Sapporo, Hokkaido 060, Japan
[5] Kyoto Univ, Dept Pediat, Kyoto 606, Japan
[6] Cent Inst Expt Anim, Kawasaki, Kanagawa, Japan
关键词
umbilical cord blood; CD34(+) cells; colony-forming cells; NOD/SCID mice; cord blood transplantation;
D O I
10.1007/BF02994007
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The characteristics of hematopoietic progenitor and stem cell (HPC/HSC) populations in mammals vary according to their ontogenic stage. In humans, HPC/HSCs from umbilical cord blood (CB) are increasingly used as an alternative to HPC/HSCs from adult bone marrow (BM) for the treatment of various hematologic disorders. How the hematopoietic activity of progenitor and stem cells in CB differs from that in adult BM remains unclear, however. We compared CD34(+) cells, a hematopoietic cell population, in CB with those in adult BM using phenotypic subpopulations analyzed by Row cytometry, the colony- forming activity in methylcellulose clonal cultures, and the repopulating ability of these: cells in NOD/Shi-scid (NOD/SCID) mice. Although the proportion of CD34(+) cells was higher in adult BM than in CB mononuclear cells, the more immature subpopulations, CD34(+)CD33(-) and CD34(+)CD38(-) cells, were present in higher proportions in CD34(+) CB cells. Clonal culture assay showed that more multipotential progenitors were present in CD34(-) CB cells. When transplanted into NOD/SCID mice, CD34(+) adult BM cells could not reconstitute human hematopoiesis in recipient BMI but CD34- CB cells achieved a high level of engraftment, indicating that CD34(+) CB cells possess a greater repopulating ability. These results demonstrated that human hematopoiesis changes with development from fetus to adult. Furthermore. CD34(+) CB cells contained a greater number of primitive hematopoietic cells, including HSCs, than did adult BM, suggesting the usefulness of CD34(+) CB cells not only as a graft for therapeutic HSC transplantation but also as a target cell population for ex vivo expansion of transplantable HSCs and for gene transfer in gene therapy. Int J Hematol. 2001;73:457-462. (C) 2001 The Japanese Society of Hematology.
引用
收藏
页码:457 / 462
页数:6
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