The PmrA/PmrB regulatory system controls the expression of the wzzfepE gene involved in the O-antigen synthesis of Salmonella enterica serovar Typhimurium

被引：25

作者：

de las Mercedes Pescaretti, Maria

Lopez, Fabian E.

Morero, Roberto D.

Delgado, Monica A. ^{[1
]}

机构：

[1] Univ Nacl Tucuman, Consejo Nacl Invest Cient & Tecn, Inst Super Invest Biol, Dept Bioquim & Nutr, RA-4000 San Miguel De Tucuman, Argentina

来源：

MICROBIOLOGY-SGM | 2011年 / 157卷

关键词：

ESCHERICHIA-COLI; SERUM RESISTANCE; CHAIN-LENGTH; RESPONSE REGULATOR; LIPOPOLYSACCHARIDE; VIRULENCE; IDENTIFICATION; MUTANTS; BIOSYNTHESIS; ENDOTOXINS;

D O I：

10.1099/mic.0.050088-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The degree of polymerization of O-antigen from Salmonella enterica serovar Typhimurium is controlled by the products of the wzz(st) and wzz(fepE) genes. In the present study we investigated the role of the PmrA/PmrB regulatory system in wzz(fepE),E transcription. We report that the direct binding of the PmrA regulator to a specific promoter site induces the expression of the wzz(fepE) gene. This effect increases the amount of very long (VL) O-antigen, which is required for the resistance of Salmonella to serum human complement and polymyxin B, and for the replication of the bacteria within macrophages. The results obtained here highlight functional differences between Wzz(fepE) and Wzz(st), although the genes for both proteins are regulated in a PmrA-dependent way.

引用

页码：2515 / 2521

页数：7

共 35 条

[1] RpoS and RpoN are involved in the growth-dependent regulation of rfaH transcription and O antigen expression in Salmonella enterica serovar typhi [J].

Bittner, M ;

Saldías, S ;

Altamirano, F ;

Valvano, MA ;

Contreras, I .

MICROBIAL PATHOGENESIS, 2004, 36 (01) :19-24

[2] Differences in gene content among Salmonella enterica serovar Typhi isolates [J].

Boyd, EF ;

Porwollik, S ;

Blackmer, F ;

McClelland, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (08) :3823-3828

[3] Growth-phase regulation of lipopolysaccharide O-antigen chain length influences serum resistance in serovars of Salmonella [J].

Bravo, Denlsse ;

Silva, Cecllia ;

Carter, Javier A. ;

Hoare, Anllei ;

Alvarez, Serglo A. ;

Blondel, Carlos J. ;

Zaldivar, Mercedes ;

Valvano, Miguel A. ;

Contreras, Ines .

JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 57 (08) :938-946

[4]

Burns SM, 1998, INFECT IMMUN, V66, P4244

[5] Fe(III)-mediated cellular toxicity [J].

Chamnongpol, S ;

Dodson, W ;

Cromie, MJ ;

Harris, ZL ;

Groisman, EA .

MOLECULAR MICROBIOLOGY, 2002, 45 (03) :711-719

[6] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].

Datsenko, KA ;

Wanner, BL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645

[7]

Davis R. W., 1980, ADV BACTERIAL GENETI

[8] Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium [J].

de las Mercedes Pescaretti, Maria ;

Morero, Roberto ;

Delgado, Monica A. .

FEMS MICROBIOLOGY LETTERS, 2009, 300 (02) :165-173

[9] The PmrA/PmrB and RcsC/YojN/RcsB systems control expression of the Salmonella O-antigen chain length determinant [J].

Delgado, MA ;

Mouslim, C ;

Groisman, EA .

MOLECULAR MICROBIOLOGY, 2006, 60 (01) :39-50

[10] MUTANTS OF SALMONELLA-TYPHIMURIUM THAT CANNOT SURVIVE WITHIN THE MACROPHAGE ARE AVIRULENT [J].

FIELDS, PI ;

SWANSON, RV ;

HAIDARIS, CG ;

HEFFRON, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (14) :5189-5193

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