Modeling fluorescence collection from single molecules in microspheres: Effects of position, orientation, and frequency

被引:32
作者
Hill, SC
Saleheen, HI
Barnes, MD
Whitten, WB
Ramsey, JM
机构
[1] NEW MEXICO STATE UNIV,DEPT ELECT & COMP ENGN,LAS CRUCES,NM 88003
[2] OAK RIDGE NATL LAB,DIV CHEM & ANALYT SCI,OAK RIDGE,TN 37831
来源
APPLIED OPTICS | 1996年 / 35卷 / 31期
关键词
fluorescence; single-molecule detection; spheres;
D O I
10.1364/AO.35.006278
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We present calculations of fluorescence from single molecules (modeled as damped oscillating dipoles) inside a dielectric sphere. For an excited molecule at an arbitrary position within the sphere we calculate the fluorescence intensity collected by an objective in some well-defined detection geometry. We find that, for the cases we model, integration over the emission linewidth of the molecule is essential for obtaining representative results. Effects such as dipole position and orientation, numerical aperture of the collection objective, sphere size, emission wavelength, and linewidth are examined. These results are applicable to single-molecule detection techniques employing microdroplets. (C) 1996 Optical Society of America
引用
收藏
页码:6278 / 6288
页数:11
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