In vivo filtering of in vitro expression data reveals MyoD targets

被引：30

作者：

Zhao, P

Seo, JW

Wang, ZY

Wang, Y

Shneiderman, B

Hoffman, EP ^{[1
]}

机构：

[1] Childrens Natl Med Ctr, Med Genet Res Ctr, Washington, DC 20010 USA

[2] Virginia Polytech Inst & State Univ, Alexandria Res Inst, Dept Elect & Comp Engn, Alexandria, VA 22314 USA

[3] Catholic Univ Amer, Dept Elect Engn & Comp Sci, Washington, DC 20064 USA

[4] Univ Maryland, Syst Res Inst, College Pk, MD 20742 USA

[5] Univ Maryland, Inst Adv Comp Studies, Human Comp Interact Lab, College Pk, MD 20742 USA

[6] Univ Maryland, Dept Comp Sci, College Pk, MD 20742 USA

来源：

COMPTES RENDUS BIOLOGIES | 2003年 / 326卷 / 10-11期

关键词：

clustering; expression profiling; microarray; muscle regeneration; MyoD;

D O I：

10.1016/j.crvi.2003.09.035

中图分类号：

Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

A published set of downstream targets of MyoD defined in a well-controlled in vitro experiment was filtered for relevance to muscle regeneration using a 27-time-point in vivo murine regeneration series. Using interactive hierarchical and Bayes soft clustering, only a minority of the targets defined in vitro can be confirmed in vivo (similar to50% of induced transcripts, and none of repressed transcripts). This approach provided strong support that 18 targets including of MyoD are biologically relevant during myoblast differentiation. (C) 2003 Academie des sciences. Published by Elsevier SAS. All rights reserved.

引用

页码：1049 / 1065

页数：17

共 20 条

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THE PROTEIN ID - A NEGATIVE REGULATOR OF HELIX-LOOP-HELIX DNA-BINDING PROTEINS [J].