Superoxide is responsible for apoptosis in rat vascular smooth muscle cells induced by α-tocopheryl hemisuccinate

被引：43

作者：

Kogure, K ^{[1
]}

Morita, M ^{[1
]}

Nakashima, S ^{[1
]}

Hama, S ^{[1
]}

Tokumura, A ^{[1
]}

Fukuzawa, K ^{[1
]}

机构：

[1] Univ Tokushima, Fac Pharmaceut Sci, Tokushima 7708505, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2001年 / 1528卷 / 01期

关键词：

alpha-tocopheryl hemisuccinate; apoptosis; superoxide; vascular smooth muscle cell;

D O I：

10.1016/S0304-4165(01)00168-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the mechanism of cell toxicity of alpha -tocopheryl hemisuccinate (TS). TS concentration- and time-dependently induced the lactate dehydrogenase release and DNA fragmentation of rat vascular smooth muscle cells (VSMC). Exogenous addition of superoxide dismutase, but not catalase, significantly inhibited the cell toxicity of TS. The NADPH-dependent oxidase activity of VSMC was stimulated by TS treatment. The cell toxicity of TS was inhibited by NADPH oxidase inhibitor 4-(2-aminoethyl)-benzenesulfonyl fluoride. Consequently, TS-induced apoptosis of VSMC was suggested to be caused by exogenous O-2(-) generated via the oxidase system activated with TS. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：25 / 30

页数：6

共 33 条

[1] NADPH oxidase: An update [J].

Babior, BM .

BLOOD, 1999, 93 (05) :1464-1476

[2]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[3] INHIBITION OF CHOLINESTERASE ACTIVITY BY TETRAHYDROAMINOACRIDINE AND THE HEMISUCCINATE ESTERS OF TOCOPHEROL AND CHOLESTEROL [J].

CHELLIAH, J ;

SMITH, JD ;

FARISS, MW .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1994, 1206 (01) :17-26

[4] Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)-benzenesulfonyl fluoride and related compounds [J].

Diatchuk, V ;

Lotan, O ;

Koshkin, V ;

Wikstroem, P ;

Pick, E .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (20) :13292-13301

[5] Growth inhibition of MCF-7 and MCF-1OA human breast cells by alpha-tocopheryl hemisuccinate, cholesteryl hemisuccinate and their ether analogs [J].

Djuric, Z ;

Heilbrun, LK ;

Lababidi, S ;

EverettBauer, CK ;

Fariss, MW .

CANCER LETTERS, 1997, 111 (1-2) :133-139

[6] THE EFFECT OF THE NADPH OXIDASE INHIBITOR DIPHENYLENEIODONIUM ON AEROBIC AND ANAEROBIC MICROBICIDAL ACTIVITIES OF HUMAN-NEUTROPHILS [J].

ELLIS, JA ;

MAYER, SJ ;

JONES, OTG .

BIOCHEMICAL JOURNAL, 1988, 251 (03) :887-891

[7]

FARISS MW, 1994, CANCER RES, V54, P3346

[8] NAD(P)H oxidase - Role in cardiovascular biology and disease [J].

Griendling, KK ;

Sorescu, D ;

Ushio-Fukai, M .

CIRCULATION RESEARCH, 2000, 86 (05) :494-501

[9] Role of oxygen radicals generated by NADPH oxidase in apoptosis induced in human leukemia cells [J].

Hiraoka, W ;

Vazquez, N ;

Nieves-Neira, W ;

Chanock, SJ ;

Pommier, Y .

JOURNAL OF CLINICAL INVESTIGATION, 1998, 102 (11) :1961-1968

[10] Vitamin E succinate induces apoptosis in human prostate cancer cells: Role for Fas in vitamin E succinate-triggered apoptosis [J].

Israel, K ;

Yu, WP ;

Sanders, BG ;

Kline, K .

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2000, 36 (01) :90-100

← 1 2 3 4 →