A program of Yersinia enterocolitica type III secretion reactions is activated by specific signals

被引:73
作者
Lee, VT [1 ]
Mazmanian, SK [1 ]
Schneewind, O [1 ]
机构
[1] Univ Calif Los Angeles, Sch Med, Dept Microbiol & Immunol, Los Angeles, CA 90095 USA
关键词
D O I
10.1128/JB.183.17.4970-4978.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Successful establishment of Yersinia infections requires the type III machinery, a protein transporter that injects virulence factors (Pops) into macrophages. It is reported here that the Yersinia type III pathway responds to environmental signals by transporting proteins to distinct locations. Yersinia enterocolitica cells sense an increase in extracellular amino acids (glutamate, glutamine, aspartate, and asparagine) that results in the activation of the type III pathway. Another signal, provided by serum proteins such as albumin, triggers the secretion of YopD into the extracellular medium. The third signal, a decrease in calcium concentration, appears to be provided by host cells and causes Y. enterocolitica to transport YopE and presumably other virulence factors across the eukaryotic plasma membrane. Mutations in several genes encoding regulatory molecules (lcrG, lcrH, tyeA, yopD, yopN yscM1, and yscM2) bypass the signal requirement of the type III pathway. Together these results suggest that yersiniae may have evolved distinct secretion reactions in response to environmental signals.
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页码:4970 / 4978
页数:9
相关论文
共 57 条
[1]   Yersinia enterocolitica type III secretion:: an mRNA signal that couples translation and secretion of YopQ [J].
Anderson, DM ;
Schneewind, O .
MOLECULAR MICROBIOLOGY, 1999, 31 (04) :1139-1148
[2]   A mRNA signal for the type III secretion of Yop proteins by Yersinia enterocolitica [J].
Anderson, DM ;
Schneewind, O .
SCIENCE, 1997, 278 (5340) :1140-1143
[3]   Dynamics of [Ca2+] in the endoplasmic reticulum and cytoplasm of intact HeLa cells - A comparative study [J].
Barrero, MJ ;
Montero, M ;
Alvarez, J .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (44) :27694-27699
[4]   ANALYSIS OF THE V-ANTIGEN LCRGVH-YOPBD OPERON OF YERSINIA-PSEUDOTUBERCULOSIS - EVIDENCE FOR A REGULATORY ROLE OF LCRH AND LCRV [J].
BERGMAN, T ;
HAKANSSON, S ;
FORSBERG, A ;
NORLANDER, L ;
MACELLARO, A ;
BACKMAN, A ;
BOLIN, I ;
WOLFWATZ, H .
JOURNAL OF BACTERIOLOGY, 1991, 173 (05) :1607-1616
[5]   Status of YopM and YopN in the Yersinia Yop virulon: YopM of Y-enterocolitica is internalized inside the cytosol of PU5-1.8 macrophages by the YopB, D, N delivery apparatus [J].
Boland, A ;
Sory, MP ;
Iriarte, M ;
Kerbourch, C ;
Wattiau, P ;
Cornelis, GR .
EMBO JOURNAL, 1996, 15 (19) :5191-5201
[6]  
BURROWS TW, 1956, BRIT J EXP PATHOL, V37, P481
[7]   LcrQ/YscM1, regulators of the Yersinia yop virulon, are injected into host cells by a chaperone-dependent mechanism [J].
Cambronne, ED ;
Cheng, LW ;
Schneewind, O .
MOLECULAR MICROBIOLOGY, 2000, 37 (02) :263-273
[8]   Type III machines of Gram-negative bacteria: delivering the goods [J].
Cheng, LW ;
Schneewind, O .
TRENDS IN MICROBIOLOGY, 2000, 8 (05) :214-220
[9]   Yersinia enterocolitica TyeA, an intracellular regulator of the type II machinery, is required for specific targeting of YopE, YopH, YopM, and YopN into the cytosol of eukaryotic cells [J].
Cheng, LW ;
Schneewind, O .
JOURNAL OF BACTERIOLOGY, 2000, 182 (11) :3183-3190
[10]   PREPARATION AND PROPERTIES OF SERUM AND PLASMA PROTEINS .4. A SYSTEM FOR THE SEPARATION INTO FRACTIONS OF THE PROTEIN AND LIPOPROTEIN COMPONENTS OF BIOLOGICAL TISSUES AND FLUIDS [J].
COHN, EJ ;
STRONG, LE ;
HUGHES, WL ;
MULFORD, DJ ;
ASHWORTH, JN ;
MELIN, M ;
TAYLOR, HL .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1946, 68 (03) :459-475