Comparison of an improved RAPD fingerprinting with different typing methods for discriminating clinical isolates of Staphylococcus spp.

被引：22

作者：

Damiani, G ^{[1
]}

Telecco, S ^{[1
]}

Comincini, S ^{[1
]}

Sironi, M ^{[1
]}

Carretto, E ^{[1
]}

Marone, P ^{[1
]}

机构：

[1] CNR,IDVGA,MILAN,ITALY

来源：

EUROPEAN JOURNAL OF EPIDEMIOLOGY | 1996年 / 12卷 / 02期

关键词：

epidemiology; RAPD; Staphylococcus spp; typing;

D O I：

10.1007/BF00145502

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Different epidemiological markers were used to characterize 2 Staphylococcus epidermidis and 8 Staphylococcus aureus strains isolated from patients with severe infections. We compared random amplified polymorphic DNA (RAPD) fingerprints, biotypes, antibiotic assays, plasmid profiles and chromosomal DNA restriction endonuclease analysis (REA). Data analysis based on numerical taxonomy methods indicates that RAPD and REA give similar results allowing a good discrimination of the two species and of each isolate. The RAPD method is easier and faster than REA, but the reproducibility of RAPD fingerprints obtained in independent experiments can be problematic. We have found simple technical devices to improve the reproducibility of the RAPD procedure which is therefore a very useful tool in epidemiology for identification and characterization of Staphylococcus spp.

引用

页码：163 / 169

页数：7

共 27 条

[1] IDENTIFICATION OF COAGULASE-NEGATIVE STAPHYLOCOCCI WITH THE API STAPH-IDENT SYSTEM
ALMEIDA, RJ
JORGENSEN, JH
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1983, 18 (02) : 254 - 257
[2] ASUBEL FM, 1987, CURRENT PROTOCOLS MO
[3] RAPID ISOLATION OF DNA FROM ACTINOMYCES
BARSOTTI, O
RENAUD, F
FRENEY, J
BENAY, G
DECORET, D
DUMONT, J
[J]. ANNALES DE L INSTITUT PASTEUR-MICROBIOLOGIE, 1987, 138 (05): : 529 - 536
[4] BINGEN E, 1994, INFECT CONT HOSP EP, V15, P488
[5] BIRNBAUM D, 1991, INFECT CONT HOSP EP, V12, P319
[6] DELENCASTRE H, 1994, EUR J CLIN MICROBIOL, V13, P64, DOI 10.1007/BF02026129
[7] FOHLF FJ, 1992, NTSYS PC VERSION 1 7
[8] MANAGEMENT AND EPIDEMIOLOGIC ANALYSES OF AN OUTBREAK DUE TO METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS
GOETZ, MB
MULLIGAN, ME
KWOK, R
OBRIEN, H
CABALLES, C
GARCIA, JP
[J]. AMERICAN JOURNAL OF MEDICINE, 1992, 92 (06) : 607 - 614
[9] TYPING OF STAPHYLOCOCCUS-AUREUS STRAINS BY PCR-AMPLIFICATION OF VARIABLE-LENGTH 16S-23S RDNA SPACER REGIONS - CHARACTERIZATION OF SPACER SEQUENCES
GURTLER, V
BARRIE, HD
[J]. MICROBIOLOGY-SGM, 1995, 141 : 1255 - 1265
[10] GENOTYPING OF STAPHYLOCOCCUS-EPIDERMIDIS BY SMALL-FRAGMENT RESTRICTION-ENDONUCLEASE ANALYSIS AND PULSED-FIELD GEL-ELECTROPHORESIS OF GENOMIC RESTRICTION FRAGMENTS
HAERTL, R
BANDLOW, G
[J]. MICROBIOLOGY AND IMMUNOLOGY, 1994, 38 (07) : 527 - 534

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