Triacylglycerol composition of Pinus koraiensis seed oil

The triacylglycerol (TG) composition of Pinus koraiensis seed oil, which contains Delta 5 nonmethylene-interrupted (NMI) fatty acids (FA) (the main acid is pinolenic, 18.3 Delta 5,9,12), was determined. TG were preliminarily separated by argentation thin-layer chromatography (TLC), and the obtained fractions were analyzed by high-temperature gas chromatography (CC) on a capillary column with methyl phenyl silicone phase. Additionally, high-performance liquid chromatography (HPLC) of TG was applied. The FA composition of all TC fractions was identified. The identification of TG was carried out by combining TLC, GC, HPLC, and calculated equivalent carbon numbers of TG standards. The TC species identification was confirmed by comparison of the theoretical recalculated and directly analyzed FA compositions of all TLC fractions of TC. Species of TC with unsaturation degrees of 1 to 7 and trace amounts of saturated and octaenoic TC species were found. Except for minor compounds, 26 TC molecular species of 32 main components were quantitatively determined. The main species were oleoyl dilinoleoylglycerol (14.7%), dilinoleoyl pinolenoylglycerol (10.7%), palmitoyl oleoyl linoleoylglycerol (8.3%), triolein (7.6%), and dioleoyl, linoleoylglycerol (7.4%). Seven TG species contained Delta 5 NMI acyl groups. Of these, the major were dilinoleoyl pinolenoylglycerol (10.7%), stearoyl linoleoyl pinolenoylglycerol (6.5%) dioleoyl, pinolenoylglycerol (5.4%), and palmitoyl linoleoyl pinolenoyl-glycerol (5.5%). TG species with two or three NMI acyl groups were not detected.