Transcriptional regulation of nicotinamide adenine dinucleotide phosphate:: quinone oxidoreductase in murine hepatoma cells by 6-(methylsufinyl)hexyl isothiocyanate, an active principle of wasabi (Eutrema wasabi Maxim)

被引：53

作者：

Hou, DX ^{[1
]}

Fukuda, M

Fujii, M

Fuke, Y

机构：

[1] Kagoshima Univ, Fac Agr, Dept Biochem Sci & Technol, Kagoshima 8900065, Japan

[2] Tokyo Metropolitan Coll, Dept Food Sci & Human Nutr, Tokyo 1968540, Japan

来源：

CANCER LETTERS | 2000年 / 161卷 / 02期

关键词：

wasabi; 6-(methylsufinyl)hexyl isothiocyanate; nicotinamide adenine dinucleotide phosphate quinone oxidoreductase; induction; reverse transcription-polymerase chain reaction transfection;

D O I：

10.1016/S0304-3835(00)00611-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Wasabi is a very popular pungent spice in Japan. This study examined the ability of 6-(methylsufinyl)hexyl isothiocyanate (6-MITC), an active principle of wasabi, to induce the cellular expression of nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase (QR) in Hepa 1c1c7 cells. The cells were treated with various concentrations of 6-MITC, and were then assessed for cell growth, QR activity and QR mRNA expression. The induction of QR activity and QR mRNA expression was time- and dose-responsive over a narrow range of 0.1-5 muM, with declining induction at higher concentrations due to cell toxicity. Furthermore, transfection studies demonstrated that the induction of transcription of the QR gene by 6-MITC involved an antioxidant/electrophile-responsive element (ARE/EpRE) activation. Our results suggest a novel mechanism by which dietary wasabi 6-MITC may be implicated in cancer chemoprevention. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：195 / 200

页数：6

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