Analysis of pregabalin at therapeutic concentrations in human plasma/serum by reversed-phase HPLC

被引：86

作者：

Berry, D ^{[1
]}

Millington, C ^{[1
]}

机构：

[1] Guys & St Thomas Trust, Med Toxicol Unit, London SE14 5ER, England

来源：

THERAPEUTIC DRUG MONITORING | 2005年 / 27卷 / 04期

关键词：

pregabalin; HPLC; assay; serum concentrations; TDM;

D O I：

10.1097/01.ftd.0000158874.54100.1a

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A selective and sensitive HPLC method is described for assay of the new antiepileptic drug pregabalin in serum/plasma. Following acetone precipitation of the sample, the drug was derivatized with picryl sulfonic acid (PSA) before chromatography on a C-8 column. No interference from coadministered antiepileptics has been observed. The assay is calibrated over the range 0.5 mg/L to 8 mg/L. In addition to qualitative and quantitative validation data for the assay, concentration measurements in predose samples from a group of patients with dose escalated to 600 mg/d pregabalin are presented. The drug concentrations measured were in the range 2.8-8.2 mg/L at steady state. The method is robust and will be suitable for monitoring pregabalin therapy.

引用

页码：451 / 456

页数：6

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