Sample preparation for peptide mapping -: A pharmaceutical quality-control perspective

被引:113
作者
Lundell, N [1 ]
Schreitmüller, T [1 ]
机构
[1] Hoffmann La Roche, PSQP, CH-4070 Basel, Switzerland
关键词
D O I
10.1006/abio.1998.2919
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In quality control of therapeutic proteins peptide mapping is used for confirmation of primary structure and detection of posttranslational modifications. The demands put on the experimental procedure are therefore different than in the case of determination of an unknown protein structure. It is here recognized that a peptide mapping method for quality control of proteins should be inert (not induce or revert modifications), general, robust, and allow a high sample throughput. The steps prior to the separation of the generated peptides are identified as crucial for meeting these demands. This includes denaturation, reduction, alkylation, buffer exchange, solubilization, and digestion. A critical review of the literature regarding these steps is presented. Relevant options in all steps are experimentally evaluated. Novel approaches are developed for many of the steps. The result is a sample preparation procedure that essentially meets the stated demands. (C) 1999 Academic Press.
引用
收藏
页码:31 / 47
页数:17
相关论文
共 113 条
[1]  
ALLEN G, 1981, LAB TECHNIQUES BIOCH
[2]   A PROCEDURE FOR INSITU ALKYLATION OF CYSTINE RESIDUES ON GLASS-FIBER PRIOR TO PROTEIN MICROSEQUENCE ANALYSIS [J].
ANDREWS, PC ;
DIXON, JE .
ANALYTICAL BIOCHEMISTRY, 1987, 161 (02) :524-528
[3]  
BAILEY JL, 1959, J BIOL CHEM, V234, P1733
[4]   THE EFFECT OF CERTAIN IONS AND OF RADIATION ON CRYSTALLINE TRYPSIN [J].
BIER, M ;
NORD, FF .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1951, 31 (02) :335-336
[5]  
BOND JS, 1989, PROTEOLYTIC ENZYMES, P232
[6]   ANALYTICAL BIOTECHNOLOGY OF RECOMBINANT PRODUCTS [J].
BORMAN, S .
ANALYTICAL CHEMISTRY, 1987, 59 (15) :A969-A973
[7]   MICROPREPARATIVE SEPARATION OF PEPTIDES DERIVED FROM SODIUM DODECYL SULFATE-SOLUBILIZED PROTEINS [J].
BOSSERHOFF, A ;
WALLACH, J ;
FRANK, RW .
JOURNAL OF CHROMATOGRAPHY, 1989, 473 (01) :71-77
[8]   USE OF 3-BROMOPROPIONIC ACID FOR DETERMINATION OF PROTEIN THIOL-GROUPS [J].
BRADBURY, AF ;
SMYTH, DG .
BIOCHEMICAL JOURNAL, 1973, 131 (04) :637-642
[9]   CARBOXYMETHYLATION OF YEAST ENOLASE [J].
BRAKE, JM ;
WOLD, F .
BIOCHEMISTRY, 1962, 1 (03) :386-&
[10]   ON MECHANISM OF ENZYME ACTION .73. STUDIES ON TRYPSINS FROM BEEF, SHEEP AND PIG PANCREAS [J].
BUCK, FF ;
VITHAYATHIL, AJ ;
NORD, FF ;
BIER, M .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1962, 97 (02) :417-&