Snail is a repressor of RK1P transcription in metastatic prostate cancer cells

被引：142

作者：

Beach, S. ^{[1
]}

Tang, H. ^{[1
]}

Park, S. ^{[1
]}

Dhillon, A. S. ^{[4
]}

Keller, E. T. ^{[2
,3
]}

Kolch, W. ^{[3
,5
]}

Yeung, K. C. ^{[1
]}

机构：

[1] Univ Toledo, Coll Med, Dept Biochem & Canc Biol, Toledo, OH 43614 USA

[2] Univ Michigan Hlth Syst, Ctr Comprehens Canc, Ann Arbor, MI USA

[3] Univ Michigan Hlth Syst, Dept Urol, Ann Arbor, MI USA

[4] Beatson Inst Canc Res, Glasgow G61 1BD, Lanark, Scotland

[5] Univ Glasgow, Sir Henry Wellcome Funct Genom Facil, Glasgow G12 8QQ, Lanark, Scotland

来源：

ONCOGENE | 2008年 / 27卷 / 15期

关键词：

regulation of RKIP expression; EMT; prostate cancer metastasis;

D O I：

10.1038/sj.onc.1210860

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diminished expression of the metastasis suppressor protein RKIP was previously reported in a number of cancers. The underlying mechanism remains unknown. Here, we show that the expression of RKIP negatively correlates with that of Snail zinc-transcriptional repressor, a key modulator of normal and neoplastic epithelial-mesenchymal transition (EMT) program. With a combination of loss-of-function and gain-of-function approaches, we showed that Snail repressed the expression of RKIP in metastatic prostate cancer cell lines. The effect of Snail on RKIP was on the level of transcriptional initiation and mediated by a proximal E-box on the RKIP promoter. Our results therefore suggest that RKIP is a novel component of the Snail transcriptional regulatory network important for the progression and metastasis of cancer.

引用

页码：2243 / 2248

页数：6

共 31 条

[1] Raf kinase inhibitor protein expression in a survival analysis of colorectal cancer patients [J].

Al-Mulla, Fahd ;

Hagan, Suzanne ;

Behbehani, Abdulla I. ;

Bitar, Milad S. ;

George, Shirley S. ;

Going, James J. ;

Curto Garcia, Jorge J. ;

Scott, Lucy ;

Fyfe, Nicky ;

Murray, Graeme I. ;

Kolch, Walter .

JOURNAL OF CLINICAL ONCOLOGY, 2006, 24 (36) :5672-5679

[2] The transcription factor Snail is a repressor of E-cadherin gene expression in epithelial tumour cells [J].

Batlle, E ;

Sancho, E ;

Franci, C ;

Domínguez, D ;

Monfar, M ;

Baulida, J ;

de Herreros, AG .

NATURE CELL BIOLOGY, 2000, 2 (02) :84-89

[3] DNA hypermethylation in tumorigenesis - epigenetics joins genetics [J].

Baylin, SB ;

Herman, JG .

TRENDS IN GENETICS, 2000, 16 (04) :168-174

[4] Correlation of Snail expression with histological grade and lymph node status in breast carcinomas [J].

Blanco, MJ ;

Moreno-Bueno, G ;

Sarrio, D ;

Locascio, A ;

Cano, A ;

Palacios, J ;

Nieto, MA .

ONCOGENE, 2002, 21 (20) :3241-3246

[5] The transcription factor Snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression [J].

Cano, A ;

Pérez-Moreno, MA ;

Rodrigo, I ;

Locascio, A ;

Blanco, MJ ;

del Barrio, MG ;

Portillo, F ;

Nieto, MA .

NATURE CELL BIOLOGY, 2000, 2 (02) :76-83

[6] RKIP sensitizes prostate and breast cancer cells to drug-induced apoptosis [J].

Chatterjee, D ;

Bai, Y ;

Wang, Z ;

Beach, S ;

Mott, S ;

Roy, R ;

Braastad, C ;

Sun, YP ;

Mukhopadhyay, A ;

Aggarwal, BB ;

Darnowski, J ;

Pantazis, P ;

Wyche, J ;

Fu, Z ;

Kitagwa, Y ;

Keller, ET ;

Sedivy, JM ;

Yeung, KC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (17) :17515-17523

[7] Unraveling signalling cascades for the Snail family of transcription factors [J].

De Craene, B ;

van Roy, F ;

Berx, G .

CELLULAR SIGNALLING, 2005, 17 (05) :535-547

[8] Delineation of prognostic biomarkers in prostate cancer [J].

Dhanasekaran, SM ;

Barrette, TR ;

Ghosh, D ;

Shah, R ;

Varambally, S ;

Kurachi, K ;

Pienta, KJ ;

Rubin, MA ;

Chinnaiyan, AM .

NATURE, 2001, 412 (6849) :822-826

[9] Effects of Raf kinase inhibitor protein expression on suppression of prostate cancer metastasis [J].

Fu, Z ;

Smith, PC ;

Zhang, LZ ;

Rubin, MA ;

Dunn, RL ;

Yao, Z ;

Keller, ET .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2003, 95 (12) :878-889

[10] Metastasis suppressor gene Raf kinase inhibitor protein (RKIP) is a novel prognostic marker in prostate cancer [J].

Fu, Z ;

Kitagawa, Y ;

Shen, RL ;

Shah, R ;

Mehra, R ;

Rhodes, D ;

Keller, PJ ;

Mizokami, A ;

Dunn, R ;

Chinnaiyan, AM ;

Yao, Z ;

Keller, ET .

PROSTATE, 2006, 66 (03) :248-256

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