Coloured Semi-reflective Thin Films for Biomass-hydrolyzing Enzyme Detection

被引：41

作者：

Cerclier, Carole ^{[1
]}

Guyomard-Lack, Aurelie ^{[1
]}

Moreau, Celine ^{[1
]}

Cousin, Fabrice ^{[2
]}

Beury, Nadege ^{[1
]}

Bonnin, Estelle ^{[1
]}

Jean, Bruno ^{[3
]}

Cathala, Bernard ^{[1
]}

机构：

[1] INRA, UR1268 Biopolymeres Interact Assemblages, F-44316 Nantes, France

[2] CEA CNRS Saclay, Lab Leon Brillouin, F-91191 Gif Sur Yvette, France

[3] Ctr Rech Macromol Veget CERMAV CNRS, F-38041 Grenoble, France

来源：

ADVANCED MATERIALS | 2011年 / 23卷 / 33期

关键词：

REDUCING SUGARS; CELLULOSE; XYLOGLUCAN; ASSAY;

D O I：

10.1002/adma.201101971

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new enzymatic activity detection assay based on colour change of the semi-reflective films is presented. The method is based on the preparation of multilayered thin films of controlled thickness obtained by sequential deposition of cellulose nanocrystals and xyloglucan. The hydrolysis of the films leads to a decrease in layer thickness that enables to detect enzyme activity, to the naked eye, from the resulting colour changes in a span of few minutes. The method allows direct, fast, highly sensitive, and easy-to-use characterization of enzymatic activities.

引用

页码：3791 / +

页数：6

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