TDP-mycaminose biosynthetic pathway revised and conversion of desosamine pathway to mycaminose pathway with one gene

被引：34

作者：

Melançon, CE

Yu, WL

Liu, HW ^{[1
]}

机构：

[1] Univ Texas, Coll Pharm, Div Med Chem, Austin, TX 78712 USA

[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 35期

关键词：

D O I：

10.1021/ja053835o

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Analysis of the tylosin gene cluster in Streptomyces fradiae uncovered an ORF, tyl1a, homologous to a hexose 3,4-isomerase found in Aneurinibacillus thermoaerophilus. Inclusion of the tyl1a gene along with other mycaminose biosynthetic genes (tylB, tylM1, tylM2, tylM3) identified in previous studies in an in vivo expression system successfully reconstituted the mycaminose pathway. Expression of tyl1a alone in the S venezuelae KdesI mutant converted a desosamine pathway to a mycaminose pathway. These results strongly support the role of Tyl1a as a TDP-4-keto-6-deoxy-D-glucose 3,4-isomerase. Copyright © 2005 American Chemical Society.

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页码：12240 / 12241

页数：2

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