Nuclear insertion sequences of mitochondrial DNA predominate in hair but not in blood of elephants

被引：69

作者：

Greenwood, AD

Pääbo, S

机构：

[1] Univ Munich, Inst Zool, D-80333 Munich, Germany

[2] Univ Munich, Max Planck Inst Evolutionary Anthropol, D-80333 Munich, Germany

来源：

MOLECULAR ECOLOGY | 1999年 / 8卷 / 01期

关键词：

elephants; ex-mitochondrial sequences; hair DNA; mitochondrial-like nuclear sequences; mtDNA; nuclear mtDNA insertions;

D O I：

10.1046/j.1365-294X.1999.00507.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hair has become a widely used source of DNA in population genetics, forensics, and conservation biology. Here we report that PCR primers that amplify a segment of the mitochondrial control region from blood DNA amplify primarily integrated nuclear copies of mitochondrial DNA from hair DNA. Thus, in some species, and under some circumstances, DNA from hair may yield unreliable results.

引用

页码：133 / 137

页数：5

共 15 条

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