Electrochemical enzyme immunoassay using model labels

被引:78
作者
Li, Xue-Mei [1 ]
Yang, Xiao-Yan [1 ]
Zhang, Shu-Sheng [1 ]
机构
[1] Qingdao Univ Sci & Technol, Key Lab Ecochem Engn, Minist Educ, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China
基金
中国国家自然科学基金;
关键词
alkaline phosphatase; ALP; EIA; electrochemical detection; enzyme immunoassay; enzyme label; glucose oxidase; GOx; horseradish peroxidase; HRP;
D O I
10.1016/j.trac.2008.04.002
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Enzyme immunoassays (EIAs) based on electrochemical detection offer several potential advantages and have been applied in clinical, medical, biotechnological, food and environmental analysis. Among the enzyme labels employed, horseradish peroxidase (HRP), alkaline phosphatase (ALP) and glucose oxidase (GOx) are the most common. This brief review reflects recent advances, challenges, and trends of electrochemical EIAs focusing on HRP, ALP or GOx as labels over the past five years. We especially emphasize current development of EIAs combined with other developments, including nanotechnology and miniaturization. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:543 / 553
页数:11
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