Reln(rl-Alb2), an allele of reeler isolated from a chlorambucil screen, is due to an IAP insertion with exon skipping

The reeler Albany2 mutation (Reln(rl-Alb2)) in the mouse is an allele of reeler isolated during a chlorambucil mutagenesis screen, Homozygous animals had drastically reduced concentrations of reelin mRNA, in which an 85-nt exon was absent, At the genomic level, the mutation was shown to be due to an intracisternal A-particle insertion leading to exon skipping, This appears to be the first observation of retrotransposon insertion during chlorambucil mutagenesis. (C) 1997 Academic Press.