Pharmacological basis for use of Lychnophora trichocarpha in gouty arthritis: Anti-hyperuricemic and anti-inflammatory effects of its extract, fraction and constituents

被引：99

作者：

de Souza, Maira Ribeiro ^{[1
]}

de Paula, Carmen Aparecida ^{[1
]}

Pereira de Resende, Michelle Luciane ^{[1
]}

Grabe-Guimaraes, Andrea ^{[2
]}

de Souza Filho, Jose Dias ^{[3
]}

Saude-Guimaraes, Denia Antunes ^{[1
]}

机构：

[1] Univ Fed Ouro Preto, Escola Farm, Lab Plantas Med, BR-35400000 Ouro Preto, MG, Brazil

[2] Univ Fed Ouro Preto, Escola Farm, Lab Farmacol Expt, BR-35400000 Ouro Preto, MG, Brazil

[3] Univ Fed Minas Gerais, Inst Ciencias Exatas, Dept Quim, Belo Horizonte, MG, Brazil

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2012年 / 142卷 / 03期

关键词：

Lychnophora trichocarpha; Flavones; Sesquiterpene lactones; Hyperuricemia; Xanthine oxidase; Gout; XANTHINE-OXIDASE ACTIVITIES; URIC-ACID LEVELS; FLAVONOIDS; DEHYDROGENASE; INHIBITION; QUERCETIN; RUTIN; MICE;

D O I：

10.1016/j.jep.2012.06.012

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Ethnopharmacological relevance: The ethanolic extract of Lychnophora trichocarpha Spreng. is used in Brazilian folk medicine to treat bruise, pain and inflammatory diseases. Aim of the study: The present study aimed at investigating whether ethanolic extract oft. trichocarpha, its ethyl acetate fraction and its main bioactive compounds could be useful to treat gouty arthritis by countering hyperuricemia and inflammation. Materials and methods: L trichocarpha ethanolic extract (LIE), ethyl acetate fraction from ethanolic extract (LTA) and isolated compounds were evaluated for urate-lowering activity and liver xanthine oxidase (XOD) inhibition in oxonate-induced hyperuricemic mice. Anti-inflammatory activity in monosodium urate crystal-induced paw oedema, an experimental model of gouty arthritis, was also investigated. Results: Crude ethanolic extract and its ethyl acetate fraction showed significant urate-lowering effects. LIE was also able to significantly inhibit liver xantine oxidase (XOD) activity in vivo at the close of 250 mg/kg. Luteolin, apigenin, lupeol, lychnopholide and eremantholide C showed the anti-hyperuricemic activities among tested compounds. Apigenin also showed XOD inhibitory activity in vivo. Luteolin, lychnopholide, lupeol and eremantholide C, in turn, did not shown significant inhibitory activity towards this enzyme, indicating that this mechanism is not likely to be involved in urate-lowering effects of those compounds. LIE, LTA, lupeol, beta-sitosterol, lychnopholide, eremantholide, luteolin and apigenin were also found to inhibit monosodium urate crystals-induced paw oedema in mice. Conclusions: Ethanolic extract of Lychnophora trichocarpha and some of its bioactive compounds may be promising agents for the treatment of gouty arthritis since they possesses both anti-hiperuricemic and anti-inflammatory properties. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：845 / 850

页数：6

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