Concentrating solutes and nanoparticles within individual aqueous microdroplets

被引：81

作者：

He, MY ^{[1
]}

Sun, CH ^{[1
]}

Chiu, DT ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 05期

关键词：

D O I：

10.1021/ac035196a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes a method to concentrate solutes and colloidal entities, from small ions and molecules to proteins and nanoparticles, within individual aqueous microdroplets in oil. The mechanism lies in the entrapment of the solutes within an aqueous microdroplet, while the water molecules from the droplet slowly dissolve into the organic phase. Because the rate of change in concentration scales as the fifth power of die surface-area-to-volume ratio of the droplet, this phenomenon is prominent mostly in the micrometer-length scale. Ibis paper presents measurements that quantify the degree of solute entrapment within the microdroplet and further describes the dynamics of droplet shrinkage and the factors that influence the rate of shrinkage. In addition, this paper explains why this concentration effect does not occur for certain organic microdroplets in aqueous solutions.

引用

收藏

页码：1222 / 1227

页数：6

相关论文

共 28 条

[1] Selective electroless and electrolytic deposition of metal for applications in microfluidics: Fabrication of a microthermocouple [J].

Allen, PB ;

Rodriguez, I ;

Kuyper, CL ;

Lorenz, RM ;

Spicar-Mihalic, P ;

Kuo, JS ;

Chiu, DT .

ANALYTICAL CHEMISTRY, 2003, 75 (07) :1578-1583

[2] Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping [J].

Anderson, JR ;

Chiu, DT ;

Jackman, RJ ;

Cherniavskaya, O ;

McDonald, JC ;

Wu, HK ;

Whitesides, SH ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 2000, 72 (14) :3158-3164

[3] DETECTION OF SINGLE RHODAMINE-6G MOLECULES IN LEVITATED MICRODROPLETS [J].

BARNES, MD ;

NG, KC ;

WHITTEN, WB ;

RAMSEY, JM .

ANALYTICAL CHEMISTRY, 1993, 65 (17) :2360-2365

[4] Probing single secretory vesicles with capillary electrophoresis [J].

Chiu, DT ;

Lillard, SJ ;

Scheller, RH ;

Zare, RN ;

Rodriguez-Cruz, SE ;

Williams, ER ;

Orwar, O ;

Sandberg, M ;

Lundqvist, JA .

SCIENCE, 1998, 279 (5354) :1190-1193

[5] Micro- and nano-scale chemical analysis of individual sub-cellular compartments [J].

Chiu, DT .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2003, 22 (08) :528-536

[6] Chemical transformations in individual ultrasmall biomimetic containers [J].

Chiu, DT ;

Wilson, CF ;

Ryttsén, F ;

Strömberg, A ;

Farre, C ;

Karlsson, A ;

Nordholm, S ;

Gaggar, A ;

Modi, BP ;

Moscho, A ;

Garza-López, RA ;

Orwar, O ;

Zare, RN .

SCIENCE, 1999, 283 (5409) :1892-1895

[7] Creating, transporting, cutting, and merging liquid droplets by electrowetting-based actuation for digital microfluidic circuits [J].

Cho, SK ;

Moon, HJ ;

Kim, CJ .

JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 2003, 12 (01) :70-80

[8]

Fredenslund A., 1977, VAPOR LIQUID EQUILIB

[9]

Geankoplis C.J., 1993, TRANSPORT PROCESSES, V3rd

[10] The effect of the hydrophobicity of microchannels and components in water and oil phases on droplet formation in microchannel water-in-oil emulsification [J].

Kawakatsu, T ;

Trägårdh, G ;

Trägårdh, C ;

Nakajima, M ;

Oda, N ;

Yonemoto, T .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2001, 179 (01) :29-37