Efficient amplification of insert end sequences from bacterial artificial chromosome clones by thermal asymmetric interlaced PCR

被引：139

作者：

Liu, YG

Huang, N

机构：

[1] S China Agr Univ, Ctr Biotechnol, Guangzhou 510642, Peoples R China

[2] Int Rice Res Inst, Manila 1099, Philippines

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1998年 / 16卷 / 02期

关键词：

bacterial artificial chromosome; chromosome walking; insert end sequences;

D O I：

10.1023/A:1007420918645

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

TAIL-PCR is a powerful tool for the recovery of DNA fragments adjacent to known sequences. A protocol is presented for the amplification of insert end sequences from bacterial artificial chromosome clones using TAIL-PCR. The amplified products are suitable as probes for chromosome walking and genome mapping and as templates for direct sequencing. The protocol has been used in rice genome studies.

引用

页码：175 / 181

页数：7

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