The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollen

被引：97

作者：

Cartagena, Joyce A. ^{[1
]}

Matsunaga, Sachihiro ^{[1
]}

Seki, Motoaki ^{[2
]}

Kurihara, Daisuke ^{[1
]}

Yokoyama, Masami ^{[1
]}

Shinozaki, Kazuo ^{[3
]}

Fujimoto, Satoru ^{[4
]}

Azumi, Yoshitaka ^{[5
]}

Uchiyama, Susumu ^{[1
]}

Fukui, Kiich ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan

[2] RIKEN, Plant Sci Ctr, Plant Funct Genom Res Grp, Plant Genom Network Res Team,Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[3] RIKEN, Plant Sci Ctr, Gene Discovery Res Grp, Gene Discovery Res Team, Tsukuba, Ibaraki 3050074, Japan

[4] Univ Wyoming, Coll Agr, Dept Mol Biol 3944, Laramie, WY 82071 USA

[5] Kanagawa Univ, Dept Biol, Kanagawa 2591293, Japan

来源：

DEVELOPMENTAL BIOLOGY | 2008年 / 315卷 / 02期

关键词：

Arabidopsis; SDG4; histone methylation; pollen tube growth; SET domain;

D O I：

10.1016/j.ydbio.2007.12.016

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plant SET domain proteins are known to be involved in the epigenetic control of gene expression during plant development. Here, we report that the Arabidopsis SET domain protein, SDG4, contributes to the epigenetic regulation of pollen tube growth, thus affecting fertilization. Using an SDG4-GFP fusion construct, the chromosomal localization of SDG4 was established in tobacco BY-2 cells. In Arabidopsis, sdg4 knockout showed reproductive defects. Tissue-specific expression analyses indicated that SDG4 is the major ASH1-related gene expressed in the pollen. Immunological analyses demonstrated that SDG4 was involved in the methylation of historic H3 in the inflorescence and pollen grains. The significant reduction in the amount of methylated historic H3 K4 and K36 in sdg4 pollen vegetative nuclei resulted in suppression of pollen tube growth. Our results indicate that SDG4 is capable of modulating the expression of genes that function in the growth of pollen tube by methylation of specific lysine residues of the histone H3 in the vegetative nuclei. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：355 / 368

页数：14

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