Opitutus terrae gen nov, sp nov, to accommodate novel strains of the division 'Verrucomicrobia' isolated from rice paddy soil

被引：150

作者：

Chin, KJ ^{[1
]}

Liesack, W ^{[1
]}

Janssen, PH ^{[1
]}

机构：

[1] Max Planck Inst Terr Mikrobiol, D-35043 Marburg, Germany

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2001年 / 51卷

关键词：

Opitutus terrae; 'Verrucomicrobia'; 16S rRNA gene; anaerobe;

D O I：

10.1099/00207713-51-6-1965

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Three strains of obligately anaerobic bacteria were isolated from rice paddy soil microcosms. Comparative analysis of the 16S rRNA genes showed that these novel isolates have identical gene sequences and are members of the division 'Verrucomicrobia'. The novel strains are phenotypically and phylogenetically distinct from species described previously. One strain, PB90-1(T), was characterized in more detail. The cells are cocci and are motile by means of a flagellum. Catalase and oxidase activities are absent. Growth-supporting substrates include mono-, di- and polysaccharides, while alcohols, amino acids and organic acids do not support growth. Propionate and acetate are the major end-products of fermentation. Nitrate is reduced to nitrite, but other external electron acceptors are not utilized. The G+C content of the genomic DNA is 74 mol%. This strain represents a taxon that has not yet been formally recognized, for which the name Opitutus terrae gem. nov., sp. nov. is proposed. The type strain is PB90-1(T) (= DSM 11246(T)).

引用

页码：1965 / 1968

页数：4

共 15 条

[1]

Chin KJ, 1999, APPL ENVIRON MICROB, V65, P5042

[2] Methanogenic degradation of polysaccharides and the characterization of polysaccharolytic clostridia from anoxic rice field soil [J].

Chin, KJ ;

Rainey, FA ;

Janssen, PH ;

Conrad, R .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1998, 21 (02) :185-200

[3] Verrucomicrobia div. nov., a new division of the bacteria containing three new species of Prosthecobacter [J].

Hedlund, BP ;

Gosink, JJ ;

Staley, JT .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1997, 72 (01) :29-38

[4]

Hengstmann U, 1999, APPL ENVIRON MICROB, V65, P5050

[5] Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity [J].

Hugenholtz, P ;

Goebel, BM ;

Pace, NR .

JOURNAL OF BACTERIOLOGY, 1998, 180 (18) :4765-4774

[6] Novel anaerobic ultramicrobacteria belonging to the Verrucomicrobiales lineage of bacterial descent isolated by dilution culture from anoxic rice paddy soil [J].

Janssen, PH ;

Schuhmann, A ;

Morschel, E ;

Rainey, FA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (04) :1382-1388

[7] Sodium-dependent succinate decarboxylation by a new anaerobic bacterium belonging to the genus Peptostreptococcus [J].

Janssen, PH ;

Liesack, W ;

Kluge, C ;

Seeliger, S ;

Schink, B ;

Harfoot, CG .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1996, 70 (01) :11-20

[8]

Jukes T. H., 1969, MAMMALIAN PROTEIN ME, P121, DOI DOI 10.1016/B978-1-4832-3211-9.50009-7

[9]

O'Farrell KA, 1999, APPL ENVIRON MICROB, V65, P4280

[10] Defensive extrusive ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia [J].

Petroni, G ;

Spring, S ;

Schleifer, KH ;

Verni, F ;

Rosati, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (04) :1813-1817

← 1 2 →