Detection of multiple rRNA-ITS regions in isolates of Ascochyta

被引：32

作者：

Fatehi, J ^{[1
]}

Bridge, P ^{[1
]}

机构：

[1] Int Mycol Inst, Egham TW20 9TY, Surrey, England

来源：

MYCOLOGICAL RESEARCH | 1998年 / 102卷

关键词：

D O I：

10.1017/S0953756297005704

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Internally transcribed spacer (ITS) regions of the rRNA gene cluster in isolates of Ascochyta spp, were amplified and digested with restriction endonucleases. Addition of fragment sizes from conventional gel electrophoresis gave anomalous sizes which suggested the presence of more than one PCR product. Separation in HA-Yellow containing agarose gel allowed the detection of fragments of the same size but with different sequences. Isolates of Ascochyta spp. were shown to possess multiple PCR-amplification products of the same sizes, but with significant sequence differences.

引用

页码：762 / 766

页数：5

共 27 条

[1] INTRASPECIFIC VARIATION WITHIN POPULATIONS OF FUSARIUM-OXYSPORUM BASED ON RFLP ANALYSIS OF THE INTERGENIC SPACER REGION OF THE RDNA [J].