Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus

被引:4
作者
Hofer, Nicole
Kolaj, Olga
Li, Hui
Cherezov, Vadim
Gillilan, Richard
Wall, J. Gerard
Caffrey, Martin [1 ]
机构
[1] Univ Limerick, Dept Chem & Environm Sci, Limerick, Ireland
[2] Univ Limerick, Mat & Surface Sci Inst, Limerick, Ireland
[3] Ohio State Univ, Dept Chem, Columbus, OH 43210 USA
[4] Cornell Univ, Cornell High Energy Synchrotron Source, Ithaca, NY 14850 USA
来源
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2007年 / 63卷
关键词
D O I
10.1107/S1744309107032277
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
CzrB is a putative zinc transporter from Thermus thermophilus. The protein is proposed to consist of a hexahelical transmembrane domain with a cytosolic extramembranal C-terminus. The latter 92-residue fragment may be expressed free and may function independently of the full-length integral membrane protein. A 6 x His-tagged form of the water-soluble fragment has been overexpressed in Escherichia coli and diffraction-quality crystals of the tagged and tag-free variants have been grown. Preliminary X-ray analyses of tag-free fragment crystals with (2.2 A resolution) and without zinc ions (1.7 A resolution) reveal that the former has at least two zinc ions bound per monomer.
引用
收藏
页码:673 / 677
页数:5
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