Two plasma membrane H+-ATPase genes are differentially expressed in iron-deficient cucumber plants

被引：122

作者：

Santi, S ^{[1
]}

Cesco, S ^{[1
]}

Varanini, Z ^{[1
]}

Pinton, R ^{[1
]}

机构：

[1] Univ Udine, Dipartimento Sci Agrarie & Ambientali, I-33100 Udine, Italy

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2005年 / 43卷 / 03期

关键词：

gene expression; H+-ATPase; Fe deficiency; plasma membrane; real-time PCR; reverse transcriptase (RT)-PCR; rhizosphere acidification;

D O I：

10.1016/j.plaphy.2005.02.007

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Aim of the present work was to investigate the involvement of plasma membrane (PM) H+-ATPase (E.C. 3.6.3.6) isoforms of cucumber (Cucumis sativus L.) in the response to Fe deficiency. Two PM H+-ATPase cDNAs (CsHA1 and CsHA2) were isolated from Cucumber and their expression analysed as a function of Fe nutritional status. Semi-quantitative reverse transcriptase (RT)-PCR and quantitative real-time RT-PCR revealed in Fe-deficient roots ail enhanced accumulation of CsHA1 gene transcripts, which were hardly detectable in leaves. Supply of iron to deficient plants caused a decrease in the transcript level of CsHA1. In contrast, CsHA2 transcripts, detected both in roots and leaves, appeared to be unaffected by Fe. This work shows for the first time that a transcriptional regulation of PM H+-ATPase involving a specific isoform occurs in the response to Fe deficiency. (c) 2005 Elsevier SAS. All rights reserved.

引用

页码：287 / 292

页数：6

共 31 条

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