Antiinflammatory effects of chiisanoside and chiisanogenin obtained from the leaves of Acanthopanax chiisanensis in the carrageenan- and Freund's complete adjuvant-induced rats

To find the antiinflammtory constituents of Acanthopanax chiisanensis (Araliaceae) leaves, phytochemical isolation procedures were performed by activity-guided fractionation in carrageenan- and Freund's complete adjuvant (FCA) reagent-induced rat models, respectively. In the two assay system. the MeOH extract (100 and 250 mg/kg, p.o.) showed significant antiinflammtory effects. Since BuOH extract among the fractionated extracts exhibited the most potent effect, it was subjected to column chromatography to yield a main triterpene glycoside, chiisanoside (1). This compound was hydrolyzed in alkaline Solution to find the biological activity of produced aglycone, chiisanogenin (1a). Oral treatment With Compounds I and la produced significant antiinflammtory effects at 10 and 30 mg/kg dose, and la was more potent than 1. The antiiflammtory effects of the two compounds were Supported by the reduction of carrageenan-induced lipid peroxidation and hydroxy radical in serum. Furthermore, treatment with 1 and I a significantly reduced rheumatoid arthritis (RA) and C-reactive protein (CRP) factors in the rat induced by Freund's complete adjuvant reagent. Compounds, I and la, inhibited xanthine oxidase activity and increased superoxide dismutase (SOD). glutathione peroxidase and catalase indicating that both compounds scavenged reactive oxygen species (ROS). (C) 2004 Elsevier Ireland Ltd. All rights reserved.