Structural and dynamic properties of bacterial Type IV secretion systems (Review)

被引:87
作者
Christie, PJ [1 ]
Cascales, E [1 ]
机构
[1] Univ Texas, Houston Med Sch, Dept Microbiol & Mol Genet, Houston, TX 77030 USA
关键词
conjugation; pathogenesis; traffic ATPases; macromolecular transport; DNA transfer;
D O I
10.1080/09687860500063316
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The type IV secretion systems (T4SS) are widely distributed among the Gram-negative and - positive bacteria. These systems mediate the transfer of DNA and protein substrates across the cell envelope to bacterial or eukaryotic cells generally through a process requiring direct cell-to-cell contact. Bacteria have evolved T4SS for survival during establishment of pathogenic or symbiotic relationships with eukaryotic hosts. The Agrobacterium tumefaciens VirB/D4 T4SS and related conjugation machines serve as models for detailed mechanistic studies aimed at elucidating the nature of translocation signals, machine assembly pathways and architectures, and the dynamics of substrate translocation. The A. tumefaciens VirB/D4 T4SS are polar-localized organelles composed of a secretion channel and an extracellular T pilus. These T4SS are assembled from 11 or more subunits. whose membrane topologies, intersubunit contacts and, in some cases, 3-dimensional structures are known. Recently, powerful in vivo assays have identified C-terminal translocation signals, defined for the first time the translocation route for a DNA substrate through a type IV secretion channel, and supplied evidence that ATP energy consumption contributes to a late stage of machine morphogenesis. Together, these recent findings describe the mechanics of type IV secretion in unprecedented detail.
引用
收藏
页码:51 / 61
页数:11
相关论文
共 76 条
[1]  
ALEGRIA MC, 2004, IN PRESS J BACTERIOL
[2]   Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretion [J].
Atmakuri, K ;
Cascales, E ;
Christie, PJ .
MOLECULAR MICROBIOLOGY, 2004, 54 (05) :1199-1211
[3]   VirE2, a type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciens [J].
Atmakuri, K ;
Ding, ZY ;
Christie, PJ .
MOLECULAR MICROBIOLOGY, 2003, 49 (06) :1699-1713
[4]   Nucleotide sequences and comparison of two large conjugative plasmids from different Campylobacter species [J].
Batchelor, RA ;
Pearson, BM ;
Friis, LM ;
Guerry, P ;
Wells, JM .
MICROBIOLOGY-SGM, 2004, 150 :3507-3517
[5]   Structure-function analysis of XcpP, a component involved in general secretory pathway-dependent protein secretion in Pseudomonas aeruginosa [J].
Bleves, S ;
Gérard-Vincent, M ;
Lazdunski, A ;
Filloux, A .
JOURNAL OF BACTERIOLOGY, 1999, 181 (13) :4012-4019
[6]  
Buchanan SK, 1999, NAT STRUCT BIOL, V6, P56
[7]   The ToIQ-ToIR proteins energize ToIA and share homologies with the flagellar motor proteins MotA-MotB [J].
Cascales, E ;
Lloubès, R ;
Sturgis, JN .
MOLECULAR MICROBIOLOGY, 2001, 42 (03) :795-807
[8]   Agrobacterium VirB10, an ATP energy sensor required for type IV secretion [J].
Cascales, E ;
Christie, PJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (49) :17228-17233
[9]   Definition of a bacterial type IV secretion pathway for a DNA substrate [J].
Cascales, E ;
Christie, PJ .
SCIENCE, 2004, 304 (5674) :1170-1173
[10]   The versatile bacterial type IV secretion systems [J].
Cascales, E ;
Christie, PJ .
NATURE REVIEWS MICROBIOLOGY, 2003, 1 (02) :137-149