Carica papaya lipase:: a novel biocatalyst for the enantioselective hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester

被引：36

作者：

Chen, CC ^{[1
]}

Tsai, SW ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Dept Chem Engn, Tainan 70101, Taiwan

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2005年 / 36卷 / 01期

关键词：

Carica papaya lipase; (R,S)-naproxen 2,2,2-trifluorothioethyl ester; kinetic resolution; hydrolysis;

D O I：

10.1016/j.enzmictec.2004.07.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Carica papaya lipase (CPL) stores in the crude papain is explored as a high enantioselective biocatalyst for the hydrolysis resolution of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents. An optimal temperature of 60 degreesC for obtaining the maximum initial rate for (S)-naproxen ester in isooctane is found, where the lipase possesses high enantioselectivity with E = 122. Less hydropholic solvents of cyclohexane and MTBE than isooctane are vital on decreasing the lipase activity and enantioselectivity. Comparisons of enzyme performances for CPL and lipase MY indicate that CPL is more enantioselective and active for (S)-naproxen ester, and also more stable in water-saturated isooctane at 60 degreesC. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：127 / 132

页数：6

共 22 条

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