Noncovalent attachment of nucleotides by fluorous fluorous interactions: Application to a simple purification principle for synthetic DNA fragments

被引：42

作者：

Beller, C ^{[1
]}

Bannwarth, W ^{[1
]}

机构：

[1] Univ Freiburg, Inst Organ Chem & Biochem, D-79104 Freiburg, Germany

来源：

HELVETICA CHIMICA ACTA | 2005年 / 88卷 / 01期

关键词：

D O I：

10.1002/hlca.200490291

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a highly efficient noncovalent immobilization of DNA fragments on surfaces by fluorous-fluorous interactions. The principle was applied to a simple and straightforward purification protocol for synthetic DNA. The method is completely compatible with standard solid-phase synthesis of DNA.

引用

页码：171 / 179

页数：9

共 23 条

[1] HINDERED DIALKYLAMINO NUCLEOSIDE PHOSPHITE REAGENTS IN THE SYNTHESIS OF 2 DNA 51-MERS [J].

ADAMS, SP ;

KAVKA, KS ;

WYKES, EJ ;

HOLDER, SB ;

GALLUPPI, GR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (03) :661-663

[2]

[Anonymous], 2002, ANGEW CHEM

[3]

[Anonymous], 2000, ANGEW CHEM, DOI [10.1002/1521-3757(20001117)112:22lt

[4]

4293::AID-ANGE4293gt

[5]

3.0.CO

[6]

2-N, DOI 10.1002/1521-3757(20001117)112:22LT

[7]

4293::AID-ANGE4293GT

[8]

3.0.CO

[9]

2-N]

[10]

BANNWARTH W, 1987, CHIMIA, V41, P302

← 1 2 3 →