Resveratrol attenuates oxLDL-stimulated NADPH oxidase activity and protects endothelial cells from oxidative functional damages

被引:95
作者
Chow, Shu-Er
Hshu, Ya-Ching
Wang, Jong-Shyan
Chen, Jan-Kan
机构
[1] Chang Gung Univ, Coll Med, Dept Physiol, Tao Yuan 333, Taiwan
[2] Chang Gung Univ, Grad Inst Rehabil Sci, Ctr Gen Studies, Tao Yuan 333, Taiwan
关键词
oxidized low-density lipoprotein; nicotinamide adenine dinuckeotide phosphate oxidase; reactive oxygen species;
D O I
10.1152/japplphysiol.00881.2006
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
trans-Resveratrol (RSV) has been shown to have cardioprotective effect during ischemia-reperfusion through reactive oxygen species (ROS)-scavenging activity. Elevated ROS has been implicated in the initiation and progression of atherosclerosis. The nicotinamide adenine dinucleotide phosphate oxidase (NOX) is a major source of vascular ROS formation. In the present study, we show that exposure of vascular endothelial cells (EC) to oxidized low-density lipoproteins (oxLDL) results in elevations of NOX activity and cellular ROS levels. The oxLDL effects are effectively suppressed by RSV or astringinin (AST), either before or after oxLDL exposure. In this study, we show that RSV or AST treatment appears to suppress NOX activity by reducing the membrane association of gp91(phox) and Rac1, two protein species required for the assembly of active NOX complex. Exposure to RSV or AST protects EC from oxidative functional damages, including antiplatelet activity and mononucleocyte adhesion. In addition, ANG II-induced NOX activation is also attenuated. These results suggest that RSV or AST protects EC from oxLDL-induced oxidative stress by both direct ROS scavenging and inhibition of NOX activity.
引用
收藏
页码:1520 / 1527
页数:8
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