Viral-induced self-assembly of magnetic nanoparticles allows the detection of viral particles in biological media

被引：448

作者：

Perez, JM ^{[1
]}

Simeone, FJ ^{[1
]}

Saeki, Y ^{[1
]}

Josephson, L ^{[1
]}

Weissleder, R ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Mol Imaging Res, Boston, MA 02129 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 34期

关键词：

D O I：

10.1021/ja036409g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Monodisperse magnetic nanoparticles conjugated with virus-surface-specific antibodies self-assemble in the presence of specific viral particles to create supramolecular structures with enhanced magnetic properties, as detected by magnetic resonance methods (NMR/MRI). The observed magnetic relaxation changes that occur upon viral-induced assembly allowed for highly sensitive and selective detection of a virus in complex biological media. The developed method was shown to specifically detect adenovirus-5 and herpes simplex virus-1 at concentrations of 5 viral particles/10 μL without the need of extensive sample preparation. The applications of this new method span from high-throughput NMR detection of viruses in biological samples to potential MR imaging of viral distribution in vivo. Copyright © 2003 American Chemical Society.

引用

页码：10192 / 10193

页数：2

共 12 条

[1] High throughput magnetic resonance imaging for evaluating targeted nanoparticle probes [J].

Högemann, D ;

Ntziachristos, V ;

Josephson, L ;

Weissleder, R .

BIOCONJUGATE CHEMISTRY, 2002, 13 (01) :116-121

[2] Analysis of multiple samples using multiplex sample NMR: Selective excitation and chemical shift imaging approaches [J].

Hou, T ;

Smith, J ;

MacNamara, E ;

Macnaughtan, M ;

Raftery, D .

ANALYTICAL CHEMISTRY, 2001, 73 (11) :2541-2546

[3] High-efficiency intracellular magnetic labeling with novel superparamagnetic-tat peptide conjugates [J].

Josephson, L ;

Tung, CH ;

Moore, A ;

Weissleder, R .

BIOCONJUGATE CHEMISTRY, 1999, 10 (02) :186-191

[4] Sample concentration and separation for nanoliter-volume NMR spectroscopy using capillary isotachophoresis [J].

Kautz, RA ;

Lacey, ME ;

Wolters, AM ;

Foret, F ;

Webb, AG ;

Karger, BL ;

Sweedler, JV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (13) :3159-3160

[5] NMR tools for biotechnology [J].

Keifer, PA .

CURRENT OPINION IN BIOTECHNOLOGY, 1999, 10 (01) :34-41

[6] Ordering of quantum dots using genetically engineered viruses [J].

Lee, SW ;

Mao, CB ;

Flynn, CE ;

Belcher, AM .

SCIENCE, 2002, 296 (5569) :892-895

[7] Detection of herpes viruses in clinical samples using real-time PCR [J].

Nicoll, S ;

Brass, A ;

Cubie, HA .

JOURNAL OF VIROLOGICAL METHODS, 2001, 96 (01) :25-31

[8] Magnetic relaxation switches capable of sensing molecular interactions [J].

Perez, JM ;

Josephson, L ;

O'Loughlin, T ;

Högemann, D ;

Weissleder, R .

NATURE BIOTECHNOLOGY, 2002, 20 (08) :816-820

[9]

Wang Q, 2002, ANGEW CHEM INT EDIT, V41, P459

[10] Natural supramolecular building blocks: Cysteine-added mutants of cowpea mosaic virus [J].

Wang, Q ;

Lin, TW ;

Johnson, JE ;

Finn, MG .

CHEMISTRY & BIOLOGY, 2002, 9 (07) :813-819

← 1 2 →