The human cell line PER.C6 provides a new manufacturing system for the production of influenza vaccines

被引：157

作者：

Pau, MG ^{[1
]}

Ophorst, C ^{[1
]}

Koldijk, MH ^{[1
]}

Schouten, G ^{[1
]}

Mehtali, M ^{[1
]}

Uytdehaag, F ^{[1
]}

机构：

[1] IntroGene BV, Crucell Holland BV, NL-2333 CN Leiden, Netherlands

来源：

VACCINE | 2001年 / 19卷 / 17-19期

关键词：

PER.C6; influenza; vaccine;

D O I：

10.1016/S0264-410X(00)00508-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Influenza viruses for vaccine production are currently grown on embryonated eggs. This manufacturing system conveys many major drawbacks such as inflexibility. cumbersome down stream processing, inability of some strains to replicate on eggs to high enough yields, and selection of receptor-binding variants with reduced antigenicity. These limitations emphasize the need for a cell line-based production system that could replace eggs in the production of influenza virus vaccines in a pandemic proof fashion. Here we present the efficient propagation of influenza A and B viruses on the fully characterized and standardized human cell line PER.C6. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2716 / 2721

页数：6

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