A PCR-RFLP method for the simultaneous differentiation of three Entamoeba species

被引：10

作者：

Fontecha, Gustavo A. ^{[1
]}

Garcia, Kimberly ^{[1
]}

Mercedes Rueda, Maria ^{[1
]}

Sosa-Ochoa, Wilfredo ^{[1
]}

Lourdes Sanchez, Ana ^{[1
,2
]}

Leiva, Byron ^{[3
]}

机构：

[1] Natl Autonomous Univ Honduras UNAH, Sch Microbiol, Microbiol Res Inst, Ciudad Univ,J1,4th Fl, Tegucigalpa, Honduras

[2] Brock Univ, Dept Hlth Sci, St Catharines, ON L2S 3A1, Canada

[3] UNAN Leon, Infect Dis Res Ctr, Fac Med Sci, Dept Microbiol & Parasitol, Leon, Nicaragua

来源：

EXPERIMENTAL PARASITOLOGY | 2015年 / 151卷

关键词：

Entamoeba histolytica; E; dispar; moshkovskii; PCR-RFLP; LINKED-IMMUNOSORBENT-ASSAY; CHAIN-REACTION PCR; REAL-TIME PCR; FECAL SAMPLES; STOOL SAMPLES; HISTOLYTICA; DISPAR; MOSHKOVSKII; SPECIMENS; DIAGNOSIS;

D O I：

10.1016/j.exppara.2015.02.003

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程]; 100103 [病原生物学];

摘要：

Amoebiasis caused by Entamoeba histolytica continues to be one of the most common parasitic diseases in the developing world. Despite its relevance, due to the lack of accurate diagnostic methods, the true clinical and public health importance of this parasite remains uncertain. The aim of this study was to develop a new diagnostic tool to differentiate E. histolytica from the morphologically undistinguishable E. dispar and E. moshkovskii. We developed a specific, fast and simple PCR-RFLP method that was able to accurately differentiate experimentally-obtained restriction patterns from the three Entamoeba species. This new method could prove useful for clinical and epidemiological purposes. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：80 / 83

页数：4

共 23 条

[1]

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[2]

High prevalence of Entamoeba moshkovskii in a Tanzanian HIV population [J].