The effect of feeding system in the expression of genes related with fat metabolism in semitendinous muscle in sheep

被引：99

作者：

Dervishi, E. ^{[1
]}

Serrano, C. ^{[2
]}

Joy, M. ^{[1
]}

Serrano, M. ^{[3
]}

Rodellar, C. ^{[2
]}

Calvo, J. H. ^{[1
,4
]}

机构：

[1] CITA, Unidad Tecnol Prod Anim, Zaragoza 50059, Spain

[2] Univ Zaragoza, Fac Vet, Dpto Anat Embriol & Genet Anim, Lab Genet Bioquim LAGENBIO, E-50009 Zaragoza, Spain

[3] INIA, Dept Mejora Genet Animal, Madrid, Spain

[4] Fdn ARAID, Zaragoza, Spain

来源：

MEAT SCIENCE | 2011年 / 89卷 / 01期

关键词：

Gene expression; Fat metabolism; Feeding system; Sheep; LIPOPROTEIN-LIPASE ACTIVITY; CONJUGATED LINOLEIC-ACID; SKELETAL-MUSCLE; ADIPOSE-TISSUE; MESSENGER-RNA; PCR DATA; GROWTH; ALPHA; CARBOXYLASE; SYNTHASE;

D O I：

10.1016/j.meatsci.2011.04.003

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

The effect of feeding system on the expression of LPL, ACACA, FASN, FABP4, DGAT1, SCD, CPT1B, PRKAA2, LEP, SREBP1, PPARG, PPARA and CEBPB genes in semitendinous muscle was studied. Forty-four single born male lambs of the Rasa Aragonesa breed, allocated to four different dietary treatments, were used: grazing alfalfa, grazing alfalfa with supplement for lambs, indoor lambs with grazing ewes and drylot. Significant differences were found in the expression of genes LPL, ACACA, FASN, FABP4, CPT1B and SCD. Genes related to adipogenesis (LPL, ACACA, FASN, FABP4, and SCD) are up-regulated in the intensive groups. In grazing groups CPT1B gene expression, related to VS-oxidation process, is up-regulated. The relative expression of CPT1B was 1.54 fold higher in ALF + S, and 0.43 and 0.37 fold lower in IND- GRE and IND, respectively. The results support the hypothesis that changes in fatty acid profile due to feeding system implicate changes in the mRNA expression level of genes related with fat metabolism. Feeding strategy is an important tool to manipulate intramuscular fatty acid profile in meat through altering gene expression of enzymes related with fat metabolism. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：91 / 97

页数：7

共 52 条

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