Tel2 mediates activation and localization of ATM/Tel1 kinase to a double-strand break

被引：42

作者：

Anderson, Carol M. ^{[1
]}

Korkin, Dmitry ^{[2
,3
,4
]}

Smith, Dana L. ^{[1
]}

Makovets, Svetlana ^{[1
]}

Seidel, Jeffrey J. ^{[1
]}

Sali, Andrej ^{[2
,3
,4
]}

Blackburn, Elizabeth H. ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Biochem, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Dept Pharmaceut Sci, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA

[4] Univ Calif San Francisco, Calif Inst Quantitat Biomed Res, San Francisco, CA 94143 USA

来源：

GENES & DEVELOPMENT | 2008年 / 22卷 / 07期

关键词：

DNA damage signaling; checkpoint; Clk-2; PI3 kinase-like kinase; alpha-superhelical protein; telomere;

D O I：

10.1101/gad.1646208

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The kinases ATM and ATR (Tel1 and Mec1 in the yeast Saccharomyces cerevisiae) control the response to DNA damage. We report that S. cerevisiae Tel2 acts at an early step of the TEL1/ATM pathway of DNA damage signaling. We show that Tel1 and Tel2 interact, and that even when Tel1 protein levels are high, this interaction is specifically required for Tel1 localization to a DNA break and its activation of downstream targets. Computational analysis revealed structural homology between Tel2 and Ddc2 (ATRIP in vertebrates), a partner of Mec1, suggesting a common structural principle used by partners of phoshoinositide 3-kinase-like kinases.

引用

页码：854 / 859

页数：6

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