Hyperinfectivity of human-passaged Vibrio cholerae can be modeled by growth in the infant mouse

被引:56
作者
Alam, A
LaRocque, RC
Harris, JB
Vanderspurt, C
Ryan, ET
Qadri, F
Calderwood, SB
机构
[1] Massachusetts Gen Hosp, Div Infect Dis, Boston, MA 02114 USA
[2] Int Ctr Diarrhoeal Dis Res, Dhaka 1000, Bangladesh
[3] Harvard Univ, Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Dept Med, Boston, MA USA
[5] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA
[6] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
关键词
D O I
10.1128/IAI.73.10.6674-6679.2005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
It has previously been shown that passage of Vibrio cholerae through the human intestine imparts a transient hyperinfectious phenotype that may contribute to the epidemic spread of cholera. The mechanism underlying this human-passaged hyperinfectivity is incompletely understood, in part due to inherent difficulties in recovering and studying organisms that are freshly passed in human stool. Here, we demonstrate that passage of V cholerae through the infant mouse intestine leads to an equivalent degree of hyperinfectivity as passage through the human host. We have used this infant mouse model of host-passaged hyperinfectivity to characterize the timing and the anatomic location of the competitive advantage of mouse-passaged V cholerae as well as the contribution of three type IV pili to the phenotype.
引用
收藏
页码:6674 / 6679
页数:6
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