Antisense inhibition of Na+/Ca2+ exchange during anoxia/reoxygenation in ventricular myocytes

被引：40

作者：

Eigel, BN ^{[1
]}

Hadley, RW ^{[1
]}

机构：

[1] Univ Kentucky, Coll Med, Dept Mol & Biomed Pharmacol, Lexington, KY 40536 USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2001年 / 281卷 / 05期

关键词：

hypoxia; calcium; heart; ischemia; sodium/calcium exchanger;

D O I：

10.1152/ajpheart.2001.281.5.H2184

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This study investigated the role of the Na+/Ca2+ exchanger (NCX) in regulating cytosolic intracellular Ca2+ concentration ([Ca2+](i)) during anoxia/reoxygenation in guinea pig ventricular myocytes. The hypothesis that the NCX is the predominant mechanism mediating [Ca2+](i) overload in this model was tested through inhibition of NCX expression by an antisense oligonucleotide. Immunocytochemistry revealed that this antisense oligonucleotide, directed at the area around the start site of the guinea pig NCX1, specifically reduced NCX expression in cultured adult myocytes by 90 +/-4%. Antisense treatment inhibited evoked NCX activity by 94 +/-3% and decreased the rise in [Ca2+](i) during anoxia/reoxygenation by 95 +/-3%. These data suggest that NCX is the predominant mechanism mediating Ca2+ overload during anoxia/reoxygenation in guinea-pig ventricular myocytes.

引用

页码：H2184 / H2190

页数：7

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