Representational difference analysis and real-time PCR for strain-specific quantification of Lactobacillus sobrius sp nov.

被引：36

作者：

Konstantinov, SR ^{[1
]}

Smidt, H ^{[1
]}

de Vos, WM ^{[1
]}

机构：

[1] Wageningen Univ, Microbiol Lab, Agrotechnol & Food Sci Grp, NL-6703 CT Wageningen, Netherlands

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 11期

关键词：

D O I：

10.1128/AEM.71.11.7578-7581.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lactobacillus sobrius sp. nov., which was recently isolated from the intestine of weaning piglets, has potential probiotic properties. To follow the fate of L. sobrius strain 001(T) in dietary interventions, a novel and strain-specific quantitative detection procedure was developed. This procedure was based on the isolation of specific genomic fragments from the type strain by representational difference analysis and their detection by real-time PCR. The described strain-specific quantification approach may be used in studies aimed at tracking bacterial strains added to specific environments.

引用

页码：7578 / 7581

页数：4

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