Structure and localization of cyclosporin synthetase, the key enzyme of cyclosporin biosynthesis in Tolypocladium inflatum

被引：46

作者：

Hoppert, M

Gentzsch, C

Schörgendorfer, K

机构：

[1] Inst Mikrobiol & Genet, D-37077 Gottingen, Germany

[2] Biochem GmbH, A-6250 Kundl, Austria

来源：

ARCHIVES OF MICROBIOLOGY | 2001年 / 176卷 / 04期

关键词：

cyclosporin A; cyclosporin synthetase; Tolypocladium inflatum; compartmentation; immunoelectron microscopy;

D O I：

10.1007/s002030100324

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The electron microscopic image of native cyclosporin synthetase molecules showed large globular complexes of 25 nm in diameter, built up by smaller interconnected units. Compartmentation of cyclosporin synthetase and the functionally interconnected D-alanine racemase was revealed after sucrose density gradient centrifugation of subcellular fractions and immunoelectron microscopy. A considerable proportion of cyclosporin synthetase and D-alanine racemase was detected at the vacuolar membrane. The product cyclosporin was localized in the fungal vacuole.

引用

页码：285 / 293

页数：9

共 35 条

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