Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer

被引：203

作者：

Zhai, YJ

Sun, F

Li, XM

Pang, H

Xu, XL

Bartlam, M

Rao, ZH ^{[1
]}

机构：

[1] Tsing Hua Univ, Tsinghua Inst Biophys, Beijing 100084, Peoples R China

[2] Tsing Hua Univ, Struct Biol Lab, Beijing 100084, Peoples R China

[3] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2005年 / 12卷 / 11期

关键词：

D O I：

10.1038/nsmb999

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). We report the crystal structure of the hexadecameric nsp7-nsp8 supercomplex from the severe acute respiratory syndrome coronavirus at 2.4-angstrom resolution. nsp8 has a novel 'golf-club' fold with two conformations. The supercomplex is a unique hollow, cylinder-like structure assembled from eight copies of nsp8 and held tightly together by eight copies of nsp7. With an internal diameter of similar to 30 angstrom, the central channel has dimensions and positive electrostatic properties favorable for nucleic acid binding, implying that its role is to confer processivity on RNA-dependent RNA polymerase.

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页码：980 / 986

页数：7

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