Interferon-β therapy for malignant melanoma:: the dose is crucial for inhibition of proliferation and induction of apoptosis of melanoma cells

被引：22

作者：

Kubo, Hitomi ^{[1
]}

Ashida, Atsuko ^{[1
]}

Matsumoto, Kazuhiko ^{[1
,2
]}

Kageshita, Toshiro ^{[1
,3
]}

Yamamoto, Akifumi ^{[4
]}

Saida, Toshiaki

机构：

[1] Shinshu Univ, Sch Med, Dept Dermatol, Matsumoto, Nagano 3908621, Japan

[2] Shinshu Univ Hosp, Clin Trial Res Ctr, Matsumoto, Nagano 3908621, Japan

[3] Kumamoto Univ, Sch Med, Dept Dermatol, Kumamoto 8608111, Japan

[4] Saitama Med Univ, Int Med Ctr, Dept Skin Oncol, Yamane, Hidaka 3501298, Japan

来源：

ARCHIVES OF DERMATOLOGICAL RESEARCH | 2008年 / 300卷 / 06期

基金：

日本学术振兴会;

关键词：

interferon(IFN)-beta; human melanoma cell; apoptosis; growth inhibition;

D O I：

10.1007/s00403-008-0841-6

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

We investigated the anti-tumor effect of human interferon-beta (HuIFN-beta) against malignant melanoma. In vitro study revealed that HuIFN-beta not only inhibited proliferation of melanoma cells (seven cell lines: MM-AN, MM-BP, MM-LH, MM-RU, PM-WK, RPM-EP, RPM-MC) but also induced apoptosis in a dose dependent fashion, though the sensitivity to HuIFN-beta was different among cell lines. In addition, we administered HuIFN-beta into cutaneous metastatic lesions of melanoma and evaluated clinical and histopathological effects. Although the size of the metastatic cutaneous lesion did not change by the intralesional injection of HuIFN-beta, histopathological examination revealed apoptotic changes of melanoma cells along with dense lymphohistiocytic infiltration. The present study confirmed direct and indirect inhibitory effects of HuIFN-beta on human melanoma cells and suggests that local higher concentration of HuIFN-beta is needed to eradicate melanoma lesions.

引用

页码：297 / 301

页数：5

共 19 条

[1] Inhibition of angiogenesis and vascular tumor growth by interferon-producing cells -: A gene therapy approach [J].

Albini, A ;

Marchisone, C ;

Del Grosso, F ;

Benelli, R ;

Masiello, L ;

Tacchetti, C ;

Bono, M ;

Ferrantini, M ;

Rozera, C ;

Truini, M ;

Belardelli, F ;

Santi, L ;

Noonan, DM .

AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (04) :1381-1393

[2]

BYERS HR, 1991, AM J PATHOL, V139, P423

[3]

Chawla-Sarkar M, 2001, CLIN CANCER RES, V7, P1821

[4] IFN-β pretreatment sensitizes human melanoma cells to TRAIL/Apo2 ligand-induced apoptosis [J].

Chawla-Sarkar, M ;

Leaman, DW ;

Jacobs, BS ;

Borden, EC .

JOURNAL OF IMMUNOLOGY, 2002, 169 (02) :847-855

[5] IDENTIFICATION OF A NOVEL SERINE THREONINE KINASE AND A NOVEL 15-KD PROTEIN AS POTENTIAL MEDIATORS OF THE GAMMA-INTERFERON-INDUCED CELL-DEATH [J].

DEISS, LP ;

FEINSTEIN, E ;

BERISSI, H ;

COHEN, O ;

KIMCHI, A .

GENES & DEVELOPMENT, 1995, 9 (01) :15-30

[6]

Dong ZY, 1999, CANCER RES, V59, P872

[7]

GARBE C, 1993, MELANOMA RES, V3, P291

[8]

GARBE C, 1990, J INVEST DERMATOL, V95, P231

[9]

Hong YK, 2000, CLIN CANCER RES, V6, P3354

[10]

Horikoshi Takashi, 1995, Journal of Dermatology (Tokyo), V22, P631

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