Synthesis, characterization, in vitro bioactivity and antimicrobial activity of magnesium and nickel doped silicate hydroxyapatite

Incorporation of Mg2+, Ni2+, and SiO44- ions into hydroxyapatite (HA) structure was successfully carried out by microwave assisted wet precipitation method to furnish Ca10-x-yMgxNiy(PO4)(6-z)(SiO4)(z)(OH)(2-z)square(z). Incorporation of silicate ions into the HA structure led to the increase in HA lattice parameters, while incorporation of (Mg2+ and Ni2+) ions led to the reduction of lattice parameters and crystallite size of silicate doped HA. Optical analysis showed a distinct red-shift upon increase in Ni2+ concentration. Dissolution of samples in simulated body fluid showed that the incorporation of Mg2+, Ni2+ and SiO44- in HA structure resulted in considerable increase in the in vitro dissolution of HA. The prepared samples promoted exceptional in vitro apatite growth over 14 days with Ca/P ratio close to 1.67. The analysis of in vitro antibacterial activity against E. coli and P. aeruginosa revealed that the Ni2+ containing samples possessed good antibacterial properties. (C) 2015 Elsevier Ltd and Techna Group S.r.l. All rights reserved.