Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells

被引：78

作者：

Chen, Y ^{[1
]}

Wang, YQ ^{[1
]}

Sun, YR ^{[1
]}

Zhang, LM ^{[1
]}

Li, WB ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet, Grp 603, Beijing 100101, Peoples R China

来源：

CURRENT GENETICS | 2001年 / 39卷 / 5-6期

关键词：

algae; Chlorella ellipsoidea; highly efficient transformation; rabbit NP-1 gene;

D O I：

10.1007/s002940100205

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A highly efficient system was developed for the expression of foreign genes in Chlorella ellipsoidea cells. The effect of five promoters on the expression efficiency of beta -glucuronidase (GUS) gene was evaluated by transient expression of the UidA gene. Among these promoters, Ubiquitin-Omega was found to be the most efficient and was selected to drive the expression of foreign genes in Chlorella cells. A gene encoding the mature rabbit neutrophil peptide-1 (NP-1) was introduced into the cells. Integration of the gene for NP-1 into the Chlorella genome was confirmed by PCR and Southern blot analysis. In vitro anti-microbial testes demonstrated the expression of biologically active NP-1 by the transgenic Chlorella cells.

引用

页码：365 / 370

页数：6

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