rho, a small GTP-binding protein, is essential for Shigella invasion of epithelial cells

被引:76
作者
Watarai, M
Kamata, Y
Kozaki, S
Sasakawa, C
机构
[1] UNIV TOKYO,INST MED SCI,DEPT BACTERIOL,MINATO KU,TOKYO 108,JAPAN
[2] UNIV OSAKA PREFECTURE,COLL AGR,DEPT VET SCI,SAKAI,OSAKA 593,JAPAN
关键词
D O I
10.1084/jem.185.2.281
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Shigella, the causative agents of bacillary dysentery, are capable of invading mammalian cells that are not normally phagocytic. Uptake of bacteria by the mammalian cells is directed by bacterial factors named IpaB, IpaC, and IpaD invasins, in which Ipa invasins secreted into the bacterial environment can interact with alpha(5) beta(1) integrin. We report here that Shigella invasion of epithelial cells requires rho activity, a ras-related GTP-binding protein. The invasive capacity of Shigella flexneri for Chinese hamster ovary (CHO) cells and other epithelial cells were greatly reduced when treated with Clostridium botulinum exoenzyme C3 transferase. Conversely, uptake of bacteria by CHO cells was promoted upon microinjection of an activated rho variant, Val14RhoA. Attachment of S. flexneri to CHO cells can elicit tyrosine phosphorylation of pp125(FAK) and paxillin, localized accumulation of F-actin, vinculin, and talin, and activation of protein kinase C, which were all blocked by the treatment with C3 transferase. Our results indicate that cellula signal transduction regulated by rho is essential for Shigella invasion of epithelial cells.
引用
收藏
页码:281 / 292
页数:12
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