Immune modulatory oligonucleotides in the prevention and treatment of OVA-induced eustachian tube dysfunction in rats

被引：9

作者：

Blanks, Deidra A.

Ebert, Charles S., Jr.

Eapen, Rose P.

Zdanski, Carlton

Kandimalla, Ekambar R.

Agrawal, Sudhir

Prazma, Jiri

机构：

[1] Univ N Carolina, Sch Med, Dept Otolaryngol Head & Neck Surg, Neurosci Hosp G0412, Chapel Hill, NC 27599 USA

[2] Idera Pharmaceut Inc, Cambridge, MA 02139 USA

来源：

OTOLARYNGOLOGY-HEAD AND NECK SURGERY | 2007年 / 137卷 / 02期

关键词：

D O I：

10.1016/j.otohns.2007.02.016

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

BACKGROUND: Otitis media with effusion (OME) is often associated with allergies. Immune modulatory ofigonucleotides (IMO) mediate allergic inflammation and may therefore be efficacious in the treatment of airway inflammation. OBJECTIVE: To evaluate the role of an IMO via transtympanic mucosal application in prevention and treatment of ovalbum-ininduced OME. DESIGN: Forty brown Norway rats were divided into control and treatment groups. Eustachian tube dysfunction was evaluated by passive opening pressures, passive closing pressures, active clearance of negative pressure, and mucociliary clearance transit time. RESULTS: Rats who underwent IMO treatment required 50% less pressure to open and close the eustachian tube (P < 0.05) and were able to actively clear 50% more negative pressure than the ovalbumin-control rats (P < 0.001). The treatment rats' mucociliary clearance time was half that of the control group (P < 0.001). CONCLUSION: IMO via transtympanic application can prevent and treat allergy-induced eustachian tube dysfunction in rats. IMO may offer substantial promise in the future management of OME. (c) 2007 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.

引用

页码：321 / 326

页数：6

共 17 条

[1] Novel immunomodulatory oligonucleotides prevent development of allergic airway inflammation and airway hyperresponsiveness in asthma [J].

Agrawal, DK ;

Edwan, J ;

Kandimalla, ER ;

Yu, D ;

Bhagat, L ;

Wang, DQ ;

Agrawal, S .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2004, 4 (01) :127-138

[2] Modulation of airway inflammation by immunostimulatory CpG oligodeoxynucleotides in a murine model of allergic aspergillosis [J].

Banerjee, B ;

Kelly, KJ ;

Fink, JN ;

Henderson, JD ;

Bansal, NK ;

Kurup, VP .

INFECTION AND IMMUNITY, 2004, 72 (10) :6087-6094

[3] Role of allergy in eustachian tube blockage and otitis media with effusion: A review [J].

Bernstein, JM .

OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 1996, 114 (04) :562-568

[4] Eustachian tube, middle ear, and mastoid anatomy; Physiology, pathophysiology, and pathogenesis [J].

Bluestone, CD ;

Alper, CM ;

Buchman, CA ;

Felding, JU ;

Ghadiali, SN ;

Hebda, PA ;

Sando, I ;

Stangerup, SE ;

Swarts, JD ;

Takahashi, H .

ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 2005, 114 (01) :16-30

[5]

EBERT CS, 2006, INT J PEDIAT OTORHIN

[6]

FIREMAN P, 1997, J ALLERGY CLIN IMMUN, V99, P787

[7]

FLISBERG K, 1963, Acta Otolaryngol Suppl, V182, P57

[8] Late-phase allergy and eustachian tube dysfunction [J].

Hardy, SM ;

Heavner, SB ;

White, DR ;

McQueen, CT ;

Prazma, J ;

Pillsbury, HC .

OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 2001, 125 (04) :339-345

[9] Immunomodulatory oligonucleotides containing a cytosine-phosphate-2'-deoxy-7-deazaguanosine motif as potent toll-like receptor 9 agonists [J].

Kandimalla, ER ;

Bhagat, L ;

Li, YK ;

Yu, D ;

Wang, D ;

Cong, YP ;

Song, SS ;

Tang, JX ;

Sullivan, T ;

Agrawal, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (19) :6925-6930

[10] Divergent synthetic nucleotide motif recognition pattern: design and development of potent immunomodulatory oligodeoxyribonucleotide agents with distinct cytokine induction profiles [J].

Kandimalla, ER ;

Bhagat, L ;

Wang, DQ ;

Yu, D ;

Zhu, FG ;

Tang, J ;

Wang, H ;

Huang, P ;

Zhang, RW ;

Agrawal, S .

NUCLEIC ACIDS RESEARCH, 2003, 31 (09) :2393-2400

← 1 2 →