Development and evaluation of a dot blot analysis for the detection of white spot syndrome baculovirus (WSBV) in Penaeus monodon

Three batches of Penaeus monodon (total of 66 shrimp) were tested for the presence of WSBV by 2-step PCR, in situ hybridization and dot blot analysis using a nucleic acid probe. The results in situ hybridization and 2-step PCR were consistent and used as the standard for comparison. Except for a low number of false negatives, results of dot blot using either DNA or tissue homogenate from the gill, pleopod, eyestalk and hemolymph paralleled the results of 2-step PCR and in situ hybridization. A quick dot blot analysis was also developed, which took only 4-4.5 h to produce results. We conclude that dot blotting using tissue homogenate from the eyestalk provides a rapid, cost effective and convenient diagnostic method for WSBV detection in the field and for screening P. monodon broodstock.